| Autor: |
Camacho-Vázquez C; Red de Estudios Moleculares Avanzados, Instituto de Ecología A. C., Carretera Antigua a Coatepec 351, El Haya, Xalapa 91073, Mexico., Elizalde-Contreras JM; Red de Estudios Moleculares Avanzados, Instituto de Ecología A. C., Carretera Antigua a Coatepec 351, El Haya, Xalapa 91073, Mexico., Reyes-Soria FA; Red de Estudios Moleculares Avanzados, Instituto de Ecología A. C., Carretera Antigua a Coatepec 351, El Haya, Xalapa 91073, Mexico., Monribot-Villanueva JL; Red de Estudios Moleculares Avanzados, Instituto de Ecología A. C., Carretera Antigua a Coatepec 351, El Haya, Xalapa 91073, Mexico., Guerrero-Analco JA; Red de Estudios Moleculares Avanzados, Instituto de Ecología A. C., Carretera Antigua a Coatepec 351, El Haya, Xalapa 91073, Mexico., Juarez-Escobar J; Facultad de Biología, Universidad Veracruzana, Zona Universitaria, Xalapa 91090, Mexico., Velázquez-López O; Red de Estudios Moleculares Avanzados, Instituto de Ecología A. C., Carretera Antigua a Coatepec 351, El Haya, Xalapa 91073, Mexico., Meza-Menchaca T; Laboratorio de Genómica Humana, Facultad de Medicina, Universidad Veracruzana, Médicos y Odontólogos S/N, Col. Unidad del Bosque, Xalapa 91010, Mexico., Bojórquez-Velázquez E; Red de Estudios Moleculares Avanzados, Instituto de Ecología A. C., Carretera Antigua a Coatepec 351, El Haya, Xalapa 91073, Mexico., Zamora-Briseño JA; Red de Estudios Moleculares Avanzados, Instituto de Ecología A. C., Carretera Antigua a Coatepec 351, El Haya, Xalapa 91073, Mexico., Ramirez-Vazquez M; Unidad de Microscopía, Facultad de Medicina, Universidad Nacional Autónoma de México (UNAM), Edificio 'A' PB, Circuito Interior, Avenida Universidad 3000, Ciudad Universitaria, Coyoacán, Ciudad de México 04510, Mexico., González Barrenechea GA; Centro de Investigación Científica de Yucatán, Carretera Sierra Papacal-Chuburná Puerto, Parque Científico y Tecnológico, Mérida 97302, Mexico., Ibarra-Laclette E; Red de Estudios Moleculares Avanzados, Instituto de Ecología A. C., Carretera Antigua a Coatepec 351, El Haya, Xalapa 91073, Mexico., Ruiz-May E; Red de Estudios Moleculares Avanzados, Instituto de Ecología A. C., Carretera Antigua a Coatepec 351, El Haya, Xalapa 91073, Mexico. |
| Abstrakt: |
In recent years, avocados have gained worldwide popularity as a nutritive food. This trend is causing a rise in the production of this fruit, which is accompanied by several problems associated with monocultural practices. Despite massive economic gains, limited molecular and structural information has been generated about avocado ripening. In fact, limited studies have attempted to unravel the proteome complexity dynamics of avocado fruit. We therefore conducted a comparative proteomics study on avocado peel and pulp during the postharvest shelf life using tandem mass tag synchronous precursor selection triple-stage mass spectrometry. We identified 3161 and 1128 proteins in the peel and pulp, respectively. Peels exhibited major over-accumulation of proteins associated with water deprivation and oxidative stress, along with abscisic acid biosynthesis. Ethylene, jasmonic acid, phenylpropanoid, and flavonoid biosynthesis pathways were activated. Structurally, we observed the accumulation of lignin and a reduction in cuticular thickness, which coincides with the reduction in the levels of long-chain acyl-coenzyme A synthetase and a marginal increase in 10,16-dihydroxyhexadecanoic acid. Our study sheds light on the association of proteome modulation with the structural features of Hass avocado. Its detailed characterization will provide an alternative for better preservation during the postharvest period. |
