Nanoplastics: Immune Impact, Detection, and Internalization after Human Blood Exposure by Single-Cell Mass Cytometry.

Autor: Fusco L; ImmuneNano-lab, Department of Biomedical Sciences, University of Padua, Padua, 35131, Italy., Gazzi A; ImmuneNano-lab, Department of Biomedical Sciences, University of Padua, Padua, 35131, Italy., Giro L; ImmuneNano-lab, Department of Biomedical Sciences, University of Padua, Padua, 35131, Italy.; Immunology Center of Georgia, Augusta University, Augusta, 30912, United States., Schefer RB; Department of Environmental Systems Science, ETH Zürich, Zürich, 8092, Switzerland., D'Almeida SM; Flow Cytometry Core Facility, School of Life Sciences, EPFL, Lausanne, 1015, Switzerland., Cagliani R; ImmuneNano-lab, Department of Biomedical Sciences, University of Padua, Padua, 35131, Italy., Zoccheddu M; Immunology Center of Georgia, Augusta University, Augusta, 30912, United States.; Georgia Cancer Center Integrated Genomics core, Augusta University, Augusta, 30912, United States., Uyar R; Stem Cell Institute, Ankara University, Ankara, 06100, Turkey., Besbinar Ö; Stem Cell Institute, Ankara University, Ankara, 06100, Turkey., Çelik D; Stem Cell Institute, Ankara University, Ankara, 06100, Turkey., Yilmazer A; Stem Cell Institute, Ankara University, Ankara, 06100, Turkey.; Department of Biomedical Engineering, Faculty of Engineering, Ankara University, Ankara, 06100, Turkey., Mitrano DM; Department of Environmental Systems Science, ETH Zürich, Zürich, 8092, Switzerland., Orecchioni M; Immunology Center of Georgia, Augusta University, Augusta, 30912, United States.; Department of Pharmacology & Toxicology, Augusta University, Augusta, 30912, United States., Delogu LG; ImmuneNano-lab, Department of Biomedical Sciences, University of Padua, Padua, 35131, Italy.; Department of Biological Sciences, Khalifa University of Science & Technology, Abu Dhabi, 127788, UAE.
Jazyk: angličtina
Zdroj: Advanced materials (Deerfield Beach, Fla.) [Adv Mater] 2024 Oct 24, pp. e2413413. Date of Electronic Publication: 2024 Oct 24.
DOI: 10.1002/adma.202413413
Abstrakt: The increasing exposure to nanoplastics (NPs) raises significant concerns for human health, primarily due to their potential bioaccumulative properties. While NPs have recently been detected in human blood, their interactions with specific immune cell subtypes and their impact on immune regulation remain unclear. In this proof-of-concept study, model palladium-doped polystyrene NPs (PS-Pd NPs) are utilized to enable single-cell mass cytometry (CyTOF) detection. The size-dependent impact of carboxylate polystyrene NPs (50-200 nm) is investigated across 15 primary immune cell subpopulations using CyTOF. By taking advantage of Pd-doping for detecting PS-Pd NPs, this work evaluates their impact on human immune-cells at the single-cell level following blood exposure. This work traces PS-Pd NPs in 37 primary immune-cell subpopulations from human blood, quantifying the palladium atom count per cell by CyTOF while simultaneously assessing the impact of PS-Pd NPs on cell viability, functionality, and uptake. These results demonstrate that NPs can interact with, interfere with, and translocate into several immune cell subpopulations after exposure. In vivo distribution experiments in mice further confirmed their accumulation in immune cells within the liver, blood, and spleen, particularly in monocytes, macrophages, and dendritic cells. These findings provide valuable insights into the impact of NPs on human health.
(© 2024 The Author(s). Advanced Materials published by Wiley‐VCH GmbH.)
Databáze: MEDLINE
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