Hydrogen isotope labeling unravels origin of soil-bound organic contaminant residues in biodegradability testing.

Autor: Lennartz S; Department of Molecular Environmental Biotechnology, Helmholtz Centre for Environmental Research - UFZ, Leipzig, Germany.; Department of Environmental Science, Aarhus University, Roskilde, Denmark., Byrne HA; Department of Molecular Environmental Biotechnology, Helmholtz Centre for Environmental Research - UFZ, Leipzig, Germany.; Department of Environmental Analytical Chemistry, Helmholtz Centre for Environmental Research - UFZ, Leipzig, Germany., Kümmel S; Department of Technical Biogeochemistry, Helmholtz Centre for Environmental Research - UFZ, Leipzig, Germany., Krauss M; Department of Exposure Science, Helmholtz Centre for Environmental Research - UFZ, Leipzig, Germany., Nowak KM; Department of Technical Biogeochemistry, Helmholtz Centre for Environmental Research - UFZ, Leipzig, Germany. karolina.nowak@ufz.de.; Chair of Geobiotechnology, Institute of Biotechnology, Technische Universität Berlin, Berlin, Germany. karolina.nowak@ufz.de.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2024 Oct 24; Vol. 15 (1), pp. 9178. Date of Electronic Publication: 2024 Oct 24.
DOI: 10.1038/s41467-024-53478-w
Abstrakt: Biodegradability testing in soil helps to identify safe synthetic organic chemicals but is still obscured by the formation of soil-bound 'non-extractable' residues (NERs). Present-day methodologies using radiocarbon or stable ( 13 C, 15 N) isotope labeling cannot easily differentiate soil-bound parent chemicals or transformation products (xenoNERs) from harmless soil-bound biomolecules of microbial degraders (bioNERs). Hypothesizing a minimal retention of hydrogen in biomolecules, we here apply stable hydrogen isotope - deuterium (D) - labeling to unravel the origin of NERs. Soil biodegradation tests with D- and 13 C-labeled 2,4-D, glyphosate and sulfamethoxazole reveal consistently lower proportions of applied D than 13 C in total NERs and in amino acids, a quantitative biomarker for bioNERs. Soil-bound D thus mostly represents xenoNERs and not bioNERs, enabling an efficient quantification of xenoNERs by just measuring the total bound D. D or tritium (T) labeling could thus improve the value of biodegradability testing results for diverse organic chemicals forming soil-bound residues.
(© 2024. The Author(s).)
Databáze: MEDLINE
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