Emergence and spread of resistant and biofilm-forming Acinetobacter baumannii in critically ill COVID-19 patients.

Autor: Silva RTPD; Federal University of Pernambuco - UFPE, Department of Biochemistry, Recife, PE, Brazil., Rocha IV; Aggeu Magalhães Institute, Oswaldo Cruz Foundation - FIOCRUZ-PE, Department of Microbiology, Recife, PE, Brazil., Dantas TF; Federal University of Pernambuco - UFPE, Department of Biochemistry, Recife, PE, Brazil., Silva JDS; Federal University of Pernambuco - UFPE, Department of Biochemistry, Recife, PE, Brazil., Costa Júnior SDD; Federal University of Paraíba - UFPB, Health Sciences Center, Department of Physiology and Pathology, João Pessoa, PB, Brazil., Luz ACO; Aggeu Magalhães Institute, Oswaldo Cruz Foundation - FIOCRUZ-PE, Department of Microbiology, Recife, PE, Brazil., Moreno M; Federal University of Paraíba - UFPB, Health Sciences Center, Department of Physiology and Pathology, João Pessoa, PB, Brazil., Leal-Balbino TC; Aggeu Magalhães Institute, Oswaldo Cruz Foundation - FIOCRUZ-PE, Department of Microbiology, Recife, PE, Brazil., Araújo Lima AV; Federal University of Pernambuco - UFPE, Department of Biochemistry, Recife, PE, Brazil., Silva EGD Jr; Regional University of Cariri - URCA, Department of Biological Chemistry, Crato, CE, Brazil., Coutinho HDM; Regional University of Cariri - URCA, Department of Biological Chemistry, Crato, CE, Brazil. Electronic address: hdmcoutinho@gmail.com., Oliveira MBM; Federal University of Pernambuco - UFPE, Department of Biochemistry, Recife, PE, Brazil. Electronic address: maria.bmoliveira@ufpe.br.
Jazyk: angličtina
Zdroj: Microbial pathogenesis [Microb Pathog] 2024 Dec; Vol. 197, pp. 107078. Date of Electronic Publication: 2024 Oct 22.
DOI: 10.1016/j.micpath.2024.107078
Abstrakt: The COVID-19 pandemic has raised concerns beyond the viral infection itself. Bacterial co-infections, particularly those involving Acinetobacter baumannii, have become a significant worry in critically ill COVID-19 patients. A. baumannii is an opportunistic pathogen that can cause nosocomial infections, especially in patients with compromised immune systems. This study investigated 36 A. baumannii isolates obtained from COVID-19 patients during a concurrent outbreak. The isolates were collected over two years through routine medical requests sent to the Clinical Microbiology laboratory. Identification of the strains was confirmed through biochemical tests, the Phoenix BD® Automated Microbiology System, and MALDI-TOF Mass Spectrometry. The study assessed the antimicrobial sensitivity of the isolates, with a specific focus on resistance to the beta-lactam group as well as aminoglycosides. The presence of specific antibiotic resistance genes (bla OXA-23 , -24 , -51 and -58 , bla KPC , bla SHV , bla IMP , bla VIM , aac(6')-Ib, ant(3″)-Ia, and aph(3')-Ia) was investigated using PCR and Sanger DNA sequencing. Biofilm-forming capabilities of the isolates were also evaluated. The findings revealed diverse resistance profiles, with a high prevalence of resistant strains, including resistance to carbapenems. Genetic analysis suggested potential clonal spread of certain strains within the hospital setting. Moreover, a significant proportion of the isolates demonstrated strong biofilm-forming abilities, which can enhance persistence and antibiotic resistance. In conclusion, this study highlights the need for vigilant monitoring and targeted interventions to address bacterial co-infections in COVID-19 patients. The diversity in resistance patterns, potential clonal spread, and robust biofilm-forming abilities among A. baumannii isolates underscore the importance of addressing this issue to better manage and treat critically ill COVID-19 patients.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financialinterestsor personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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