Safety evaluations for transtympanic laser stimulation of the cochlea in Mongolian gerbils (Meriones unguiculatus).

Autor: Okamoto A; Neuroethology and Bioengineering, Graduate School of Life and Medical Sciences, Doshisha University, Kyotanabe, Kyoto, Japan., Uenaka M; Neuroethology and Bioengineering, Graduate School of Life and Medical Sciences, Doshisha University, Kyotanabe, Kyoto, Japan., Ito Y; Neuroethology and Bioengineering, Graduate School of Life and Medical Sciences, Doshisha University, Kyotanabe, Kyoto, Japan., Kuroki Y; Neuroethology and Bioengineering, Graduate School of Life and Medical Sciences, Doshisha University, Kyotanabe, Kyoto, Japan., Miyasaka T; Neuropathology, Graduate School of Life and Medical Sciences, Doshisha University, Kyotanabe, Kyoto, Japan., Toda K; Department of Psychology, Keio University, Tokyo, Japan., Hiryu S; Neuroethology and Bioengineering, Graduate School of Life and Medical Sciences, Doshisha University, Kyotanabe, Kyoto, Japan., Kobayasi KI; Neuroethology and Bioengineering, Graduate School of Life and Medical Sciences, Doshisha University, Kyotanabe, Kyoto, Japan. Electronic address: kkobayas@mail.doshisha.ac.jp., Tamai Y; Neuroethology and Bioengineering, Graduate School of Life and Medical Sciences, Doshisha University, Kyotanabe, Kyoto, Japan; Neurobiology of Social Communication, Department of Otolaryngology-Head and Neck Surgery, Hearing Research Centre, University of Tübingen, Medical Center, Elfriede-Aulhorn-Strasse 5, Tübingen 72076, Germany. Electronic address: ytamai@mail.doshisha.ac.jp.
Jazyk: angličtina
Zdroj: Neuroscience research [Neurosci Res] 2024 Oct 22. Date of Electronic Publication: 2024 Oct 22.
DOI: 10.1016/j.neures.2024.10.004
Abstrakt: Infrared laser stimulation of the cochlea has been proposed as a possible alternative to conventional auditory prostheses. Whereas previous studies have focused primarily on the short-term effects of laser stimulation, the practical application of this technics requires an investigation into whether prolonged laser exposure can induce neural responses and safely. This study assessed the effect of laser-induced damage to the cochlea on auditory perception using Mongolian gerbils (Meriones unguiculatus) trained with a classical conditioning task. The broadband noise was presented as a conditioned stimulus, and reward licking was recorded as a conditioned response. After training, the subject's cochlea was exposed to a continuous pulsed laser for 15 h. Broadband noise of various intensities was presented without pairing it with water before and after laser exposure to assess the decrease in auditory perception due to laser-induced injury. The licking rate did not change after laser exposure of 6.6 W/cm 2 or weaker but drastically decreased after 26.4 W/cm 2 or higher. These findings showed, for the first time, that the safety margin of long-term, at least several hours, cochlear laser stimulation exists and will contribute to the appropriate delimitation of the safe and effective laser stimulation parameters in future research.
Competing Interests: Declaration of Competing Interest The authors have no conflicts of interest to declare.
(Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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