Generation of IPi002-A/B/C human induced pluripotent stem cell lines from MARCH amniotic fluid cells.

Autor:	Boullé M; Chemogenomic and Biological Screening Core Facility, Center for Technological Resources and Research, Department of Structural Biology and Chemistry, Institut Pasteur, Université Paris-Cité, CNRS UMR 3523, F-75015 Paris, France. Electronic address: mikael.boulle@pasteur.fr., Leleu A; SupBiotech-CEA/DRF/IBFJ/SEPIA, 92260 Fontenay-aux-Roses, France., Schacre S; Chemogenomic and Biological Screening Core Facility, Center for Technological Resources and Research, Department of Structural Biology and Chemistry, Institut Pasteur, Université Paris-Cité, CNRS UMR 3523, F-75015 Paris, France., Banal C; Université Paris-Cité, iPSC Core Facility, Institut Imagine, INSERM UMR U1163, 75015 Paris, France., Boucharlat A; Chemogenomic and Biological Screening Core Facility, Center for Technological Resources and Research, Department of Structural Biology and Chemistry, Institut Pasteur, Université Paris-Cité, CNRS UMR 3523, F-75015 Paris, France., Renault S; SupBiotech-CEA/DRF/IBFJ/SEPIA, 92260 Fontenay-aux-Roses, France., Hollenstein M; Laboratory for Bioorganic Chemistry of Nucleic Acids, Department of Structural Biology and Chemistry, Institut Pasteur, Université Paris-Cité, CNRS UMR 3523, F-75015 Paris, France., Frosk P; Department of Pediatrics and Child Health, Department of Biochemistry and Medical Genetics, Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba, Winnipeg, Manitoba, Canada., Yates F; SupBiotech-CEA/DRF/IBFJ/SEPIA, 92260 Fontenay-aux-Roses, France., Lefort N; Université Paris-Cité, iPSC Core Facility, Institut Imagine, INSERM UMR U1163, 75015 Paris, France., Agou F; Chemogenomic and Biological Screening Core Facility, Center for Technological Resources and Research, Department of Structural Biology and Chemistry, Institut Pasteur, Université Paris-Cité, CNRS UMR 3523, F-75015 Paris, France. Electronic address: fabrice.agou@pasteur.fr.
Jazyk:	angličtina
Zdroj:	Stem cell research [Stem Cell Res] 2024 Dec; Vol. 81, pp. 103589. Date of Electronic Publication: 2024 Oct 19.
DOI:	10.1016/j.scr.2024.103589
Abstrakt:	Human induced pluripotent stem cells (hiPSCs) have become a revolutionary tool in biomedical research due to their unique in vitro properties and fate versatility. They offer insights into development or genetic disorders, facilitate drug discovery and hold promise for regenerative medicine. Here we generated three hiPSC cells - IPi002-A/B/C - from primary amniotic fluid cells (AFCs) obtained via amniocentesis for the prenatal diagnosis of MARCH syndrome: Multinucleated neurons, Anhydramnios, Renal dysplasia, Cerebellar hypoplasia, and Hydranencephaly. These AFCs underwent reprogramming through non-integrative viral transduction and the resulting hiPSCs exhibited normal karyotype and expressed typical pluripotency markers. Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Fabrice Agou and Mikael Boulle reports financial support was provided by Finnish Academy of Science and Letters. Fabrice Agou reports financial support was provided by French National Research Agency. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)
Databáze:	MEDLINE
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