Eosinophil-Airway Epithelial Cells crosstalk reveals the eosinophils-mediated DUOX1 up-regulation in a murine allergic inflammation setting.

Autor:	Raggi C; Department of Oncology and Molecular Medicine, Istituto Superiore di Sanità, Rome, Italy., Spadaro F; Core Facilities-Confocal microscopy Unit, Istituto Superiore di Sanità, Rome, Italy., Mattei F; Department of Oncology and Molecular Medicine, Istituto Superiore di Sanità, Rome, Italy., Gambardella AR; Department of Oncology and Molecular Medicine, Istituto Superiore di Sanità, Rome, Italy., Noto F; Department of Oncology and Molecular Medicine, Istituto Superiore di Sanità, Rome, Italy., Andreone S; Department of Oncology and Molecular Medicine, Istituto Superiore di Sanità, Rome, Italy., Signore M; Core Facilities-Proteomics, RPPA Unit, Istituto Superiore di Sanità, Rome, Italy., Schiavoni G; Department of Oncology and Molecular Medicine, Istituto Superiore di Sanità, Rome, Italy., Parolini I; Department of Oncology and Molecular Medicine, Istituto Superiore di Sanità, Rome, Italy.; Laboratory of Molecular Biology and DNA repair, Department of Medicine (DMED), University of Udine, Udine, Italy., Afferni C; National Center for Drug Research and Evaluation, Istituto Superiore di Sanità, Rome, Italy.
Jazyk:	angličtina
Zdroj:	Journal of leukocyte biology [J Leukoc Biol] 2024 Oct 24. Date of Electronic Publication: 2024 Oct 24.
DOI:	10.1093/jleuko/qiae232
Abstrakt:	Blood and airway eosinophilia represent markers for the endotype-driven treatment of allergic asthma. Little is known on mechanisms that link eosinophils and airway epithelial cells before and after these cells are infiltrated by eosinophils during allergic response. Given that innate immune mechanisms, mainly mediated by epithelial-derived cytokines (IL-33, IL-25, TSLP), induce eosinophil-maturing/attractive substances, we thought to evaluate the crosstalk between eosinophils and airway epithelial cells in the context of IL-33-mediated allergic inflammation. DUOX1 was previously described in clinically relevant aspects of allergic inflammation in a HDM -induced allergic asthma mice model, and in patients with chronic sinusitis or allergic asthma. Thus, we evaluated the involvement of HDM and eosinophils in the regulation of DUOX1 in airway epithelial cells. To recapitulate the lung environment present at the allergen challenge time in acute asthma, we set up an in vitro model based on murine bone marrow-derived eosinophils differentiated with IL-5 and then activated with IL-33 (EOs33) and TC1 or C57 airway epithelial cells. We found that treatment of epithelial cells with HDM induced an eosinophil-attractive environment and increased DUOX1 expression. Importantly, we found that the co-culture of airway epithelial cells with EOs33 or with conditioned medium from EOs33 enhanced the expression of DUOX1, which was further increased by combined stimulation (HDM plus EOs33). Our results suggest that lung recruited EOs once activated by IL-33 could be involved in a crosstalk loop with airway epithelial cells by DUOX1-mediated IL-33 secretion. (© The Author(s) 2024. Published by Oxford University Press on behalf of Society for Leukocyte Biology.)
Databáze:	MEDLINE
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