Silver nanoparticles incorporated dental restorative resin and its antibiofilm effect.

Autor:	Campos-Ibarra V; Interdisciplinary Research Laboratory (LII), Nanostructures and Biomaterials Area, Escuela Nacional de Estudios Superiores Unidad León, Universidad Nacional Autónoma de México, León 37689, Mexico., Rodríguez-Moreno A; Faculty of Stomatology, UASLP, San Luis Potosi, Mexico., Zavala-Alonso NV; Faculty of Stomatology, UASLP, San Luis Potosi, Mexico., Vargas-Sanchez LO; Biochemical and Microbiology Laboratory, Faculty of Stomatology, UASLP, San Luis Potosi, Mexico., Loredo-Tobias M; Área de Ciencias de la Tierra, Facultad de Ingeniería, UASLP, San Luis Potosí, Mexico., García-Arreola ME; Área de Ciencias de la Tierra, Facultad de Ingeniería, UASLP, San Luis Potosí, Mexico., Aranda-Herrera B; Interdisciplinary Research Laboratory (LII), Nanostructures and Biomaterials Area, Escuela Nacional de Estudios Superiores Unidad León, Universidad Nacional Autónoma de México, León 37689, Mexico., Ruiz-Garcia J; Biological Physics Laboratory, Physics Institute, UASLP, San Luis Potosi, Mexico., Manisekaran R; Interdisciplinary Research Laboratory (LII), Nanostructures and Biomaterials Area, Escuela Nacional de Estudios Superiores Unidad León, Universidad Nacional Autónoma de México, León 37689, Mexico.
Jazyk:	angličtina
Zdroj:	Royal Society open science [R Soc Open Sci] 2024 Sep 18; Vol. 11 (9), pp. 240915. Date of Electronic Publication: 2024 Sep 18 (Print Publication: 2024).
DOI:	10.1098/rsos.240915
Abstrakt:	Dental restoration materials are susceptible to bacterial biofilm formation, which damages the restorations and causes oral health problems. Therefore, to overcome this, silver nanoparticles (AgNPs) are studied widely due to their antimicrobial, anti-inflammatory and healing properties. The purpose of this study was to develop a strategy for incorporating AgNPs onto the surface of bisacrylic resin (Bis) to evaluate its antibiofilm effects using Streptococcus sanguinis and Actinomyces naeslundii . AgNPs with an average size of 25 nm at two different concentrations were dispersed on the Bis surface (Bis-AgNPs) by mechanical deposition. Ag release was quantified until 7 days of incubation. Bacterial growth was assessed using a viability assay kit and observed using confocal microscopy. The biofilm biomass was quantified using arbitrary fluorescence units. Cell viability was evaluated using an MTT assay. The results showed that Bis-AgNPs significantly inhibited biofilm formation along with a significant difference in the viability of human gingival fibroblasts. The quantification confirmed a decrease in Ag release over time, and elemental mapping showed AgNP penetration up to 10 µm from the surface. Therefore, it was concluded that Bis-AgNPs presented enhanced antibiofilm properties, even at a concentration with no adverse effects. Therefore, this nanocomposite may be a promising alternative for biofilm control in temporary restorative materials. Competing Interests: We declare we have no competing interests. (© 2024 The Author(s).)
Databáze:	MEDLINE
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