Association of Fusobacterium nucleatum Levels by ddPCR in Oral Rinse Samples With Periodontal Disease in Oral Squamous Cell Carcinoma Patients and in Controls.

Autor:	Fernandes BB; Department of Oral Cancer Prevention, Barretos Cancer Hospital, Barretos, Brazil.; Department of Dentistry, Barretos Cancer Hospital, Barretos, Brazil., Datorre JG; Molecular Oncology Research Center, Barretos Cancer Hospital, Barretos, Brazil., Vazquez FL; Department of Oral Cancer Prevention, Barretos Cancer Hospital, Barretos, Brazil.; Department of Dentistry, Barretos Cancer Hospital, Barretos, Brazil., Peters ACC; Molecular Oncology Research Center, Barretos Cancer Hospital, Barretos, Brazil., Coracin FL; Department of Oral Cancer Prevention, Barretos Cancer Hospital, Barretos, Brazil.; Department of Dentistry, Barretos Cancer Hospital, Barretos, Brazil., Reis RM; Molecular Oncology Research Center, Barretos Cancer Hospital, Barretos, Brazil.; Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, Braga, Portugal.; ICVS/3B's-PT Government Associate Laboratory, Braga/Guimarães, Portugal., Arantes LMRB; Molecular Oncology Research Center, Barretos Cancer Hospital, Barretos, Brazil., Gama RR; Head and Neck Surgery Department, Barretos Cancer Hospital, Barretos, Brazil.
Jazyk:	angličtina
Zdroj:	Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology [J Oral Pathol Med] 2024 Nov; Vol. 53 (10), pp. 657-666. Date of Electronic Publication: 2024 Oct 23.
DOI:	10.1111/jop.13580
Abstrakt:	Background: The role of microbiome, particularly Fusobacterium nucleatum (Fn), in periodontal disease and oral squamous cell carcinoma (OSCC) has been recently explored. This study aimed to evaluate the Fn presence and its levels in oral rinse samples from Brazilian OSCC patients and healthy individuals and its association with sociodemographic, clinical, and oral health features. Methods: In this case-control study, 80 participants were included, 31 OSCC patients and 49 individuals without a cancer history. Clinical data were collected, and an oral exam was done on a subset of the cohort. Fn levels were evaluated by droplet digital PCR (ddPCR) in oral rinse samples and were categorized as Fn-high or Fn-low based on the median number of copies per reaction. Results: OSCC patients showed higher levels of Fn (68%, p = 0.03) than controls, and all OSCC cases were diagnosed with periodontal disease (100%, p = 1.0). In the univariate analysis, Fn-high level was more frequently present in OSCC cases compared to controls (p = 0.01). It was also observed that Fn-high level OSCC cases were significantly associated with self-reported non-white ethnicity (71.4%, p = 0.01) and had more infiltrative lesions (57.1%, p = 0.02) than Fn-low OSCC cases. Fn-high levels in oral rinse samples, were significantly more prevalent among OSCC than in controls. Conclusions: In OSCC patients, Fn-high levels were associated with non-white ethnicity and lesions with infiltrative clinical aspects. Among OSCC cases, all had periodontal disease. (© 2024 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
Databáze:	MEDLINE
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