| Autor: |
Caselli L; Department of Physical Chemistry 1, Lund University, Lund SE-22100, Sweden., Du G; Department of Physical Chemistry 1, Lund University, Lund SE-22100, Sweden., Micciulla S; Institut Laue-Langevin, CS 20156, Grenoble, Cedex 9 38042, France., Traini T; Department of Pharmacy, University of Copenhagen, Copenhagen DK-2100, Denmark., Sebastiani F; Department of Physical Chemistry 1, Lund University, Lund SE-22100, Sweden.; Department of Pharmacy, University of Copenhagen, Copenhagen DK-2100, Denmark., Diedrichsen RG; Department of Pharmacy, University of Copenhagen, Copenhagen DK-2100, Denmark., Köhler S; LINXS Institute of Advanced Neutron and X-ray Science, Scheelevagen 19, Lund 22370, Sweden., Skoda MWA; ISIS Pulsed Neutron and Muon Source, Rutherford Appleton Laboratory, Harwell OX11 0QX, U.K., van der Plas MJA; Department of Pharmacy, University of Copenhagen, Copenhagen DK-2100, Denmark., Malmsten M; Department of Physical Chemistry 1, Lund University, Lund SE-22100, Sweden.; Department of Pharmacy, University of Copenhagen, Copenhagen DK-2100, Denmark. |
| Abstrakt: |
In this study, we report the degradation of smooth and rough lipopolysaccharides (LPS) from Gram-negative bacteria and of lipoteichoic acid (LTA) from Gram-positive bacteria by peptide-coated TiO 2 nanoparticles (TiO 2 NPs). While bare TiO 2 NPs displayed minor binding to both LPS and LTA, coating TiO 2 NPs with the antimicrobial peptide LL-37 dramatically increased the level of binding to both LPS and LTA, decorating these uniformly. Importantly, peptide coating did not suppress reactive oxygen species generation of TiO 2 NPs; hence, UV illumination triggered pronounced degradation of LPS and LTA by peptide-coated TiO 2 NPs. Structural consequences of oxidative degradation were examined by neutron reflectometry for smooth LPS, showing that degradation occurred preferentially in its outer O-antigen tails. Furthermore, cryo-TEM and light scattering showed lipopolysaccharide fragments resulting from degradation to be captured by the NP/lipopolysaccharide coaggregates. The capacity of LL-37-TiO 2 NPs to capture and degrade LPS and LTA was demonstrated to be of importance for their ability to suppress lipopolysaccharide-induced activation in human monocytes at simultaneously low toxicity. Together, these results suggest that peptide-coated photocatalytic NPs offer opportunities for the confinement of infection and inflammation. |
