Ultrasound-assisted low-temperature extraction of polysaccharides from Lavandula angustifolia Mill.: optimization, structure characterization, and anti-inflammatory activity.

Autor: Cao L; Key Lab of Natural Product Chemistry and Application, School of Chemistry and Chermical Engineering, Yili Normal University, Yining 835000, China., Liu W; Key Lab of Natural Product Chemistry and Application, School of Chemistry and Chermical Engineering, Yili Normal University, Yining 835000, China., Jing H; Xinjiang eprhan Spice Co., Ltd. No, Kekedala 835219, China., Yasen A; Key Lab of Natural Product Chemistry and Application, School of Chemistry and Chermical Engineering, Yili Normal University, Yining 835000, China., Wang J; Key Lab of Natural Product Chemistry and Application, School of Chemistry and Chermical Engineering, Yili Normal University, Yining 835000, China., Wang Y; Key Lab of Natural Product Chemistry and Application, School of Chemistry and Chermical Engineering, Yili Normal University, Yining 835000, China., Yang Z; Key Lab of Natural Product Chemistry and Application, School of Chemistry and Chermical Engineering, Yili Normal University, Yining 835000, China., Yili A; Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi 830011, China., Nuerxiati R; Key Lab of Natural Product Chemistry and Application, School of Chemistry and Chermical Engineering, Yili Normal University, Yining 835000, China. Electronic address: rehebatinuerxiati17@mails.ucas.ac.cn., Weng Z; School of Chinese Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China. Electronic address: wengzebin@njucm.edu.cn.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2024 Dec; Vol. 282 (Pt 2), pp. 136764. Date of Electronic Publication: 2024 Oct 21.
DOI: 10.1016/j.ijbiomac.2024.136764
Abstrakt: Lavandula angustifolia Mill. is a traditional Chinese medicinal herb with high economic and pharmacological value. In this study, we optimized the conditions for low-temperature ultrasound-assisted extraction of L. angustifolia Mill. polysaccharides using response surface methodology (RSM), and two homopolysaccharides LAPW1 (33.6 kDa) and LAPS1 (95.9 kDa) were obtained after isolation and purification by DEAE-650 M and Superdex™ 200 chromatography. The primary structure of LAPS1 was determined using "partial acid hydrolysis, methylation, two-dimensional (2D NMR) spectroscopy" as the core method. The results revealed that LAPS1 is a heteropolysaccharide whose main chain consists of [-1)-β-Galp-(6→1)-α-Araf-(5→] 3 -1-α-Araf-(3→1)-α-Araf-(5→[1)-β-Galp-(6→1)-α-Araf-(5→] 3 -1-α-Araf-(5→1)-α-Araf-(5→[-1)-β-Galp-(6→1)-α-Araf-(5→] 3 . In vitro experiments revealed that LAPW1 and LAPS1 significantly reduced the production of the inflammatory cytokines Interleukin-1β (IL-1β), Interleukin-6 (IL-6), and Tumor Necrosis Factor (TNF), as well as the expression of Nitric Oxide Synthase 2 (NOS2) and release of nitric oxide ( . NO) free radical in the inflammatory model established by lipopolysaccharide (LPS) stimulated RAW264.7. Besides, the zebrafish inflammatory model, stimulated by CuSO 4 , was employed to assess the impact of polysaccharides on neutrophil migration, indicating a notable decrease in zebrafish neutrophils and confirming their potential anti-inflammatory activity. These results indicate that polysaccharides from L. angustifolia Mill. be used in the development of functional foods and pharmaceutical products.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: