Metataxonomy and pigments analyses unravel microbial diversity and the relevance of retinal-based photoheterotrophy at different salinities in the Odiel Salterns (SW, Spain).

Autor: Gómez-Villegas P; Laboratory of Biochemistry, Center for Natural Resources, Health and Environment (RENSMA), University of Huelva, 21071 Huelva, Spain., Pérez-Rodríguez M; Department of Cell Biology, Physiology and Immunology, University of Córdoba, Campus de Excelencia Internacional Agroalimentario CeiA3, Córdoba, Spain., Porres JM; Department of Physiology, Institute of Nutrition and Food Technology (INyTA), Biomedical Research Center (CIBM), Universidad de Granada, 18100 Granada, Spain., Prados JC; Department of Anatomy and Embryology, Faculty of Medicine, Institute of Biopathology and Regenerative Medicine (IBIMER), Biomedical Research Center (CIBM), Universidad de Granada, 18100 Granada, Spain., Melguizo C; Department of Anatomy and Embryology, Faculty of Medicine, Institute of Biopathology and Regenerative Medicine (IBIMER), Biomedical Research Center (CIBM), Universidad de Granada, 18100 Granada, Spain., Vigara J; Laboratory of Biochemistry, Center for Natural Resources, Health and Environment (RENSMA), University of Huelva, 21071 Huelva, Spain., Moreno-Garrido I; Institute of Marine Sciences of Andalusia (CSIC), Campus Univ. Río San Pedro, Puerto Real, 11519 Cádiz, Spain., León R; Laboratory of Biochemistry, Center for Natural Resources, Health and Environment (RENSMA), University of Huelva, 21071 Huelva, Spain. Electronic address: rleon@uhu.es.
Jazyk: angličtina
Zdroj: Journal of photochemistry and photobiology. B, Biology [J Photochem Photobiol B] 2024 Nov; Vol. 260, pp. 113043. Date of Electronic Publication: 2024 Oct 17.
DOI: 10.1016/j.jphotobiol.2024.113043
Abstrakt: Salinity has a strong influence on microorganisms distribution patterns and consequently on the relevance of photoheterotrophic metabolism, which since the discovery of proteorhodopsins is considered the main contributor to solar energy capture on the surface of the oceans. Solar salterns constitute an exceptional system for the simultaneous study of several salt concentrations, ranging from seawater, the most abundant environment on Earth, to saturated brine, one of the most extreme, which has been scarcely studied. In this study, pigment composition across the salinity gradient has been analyzed by spectrophotometry and RP-HPLC, and the influence of salinity on microbial diversity of the three domains of life has been evaluated by a metataxonomic study targeting hypervariable regions of 16S and 18S rRNA genes. Furthermore, based on the chlorophyll a and retinal content, we have estimated the relative abundance of rhodopsins and photosynthetic reaction centers, concluding that there is a strong correlation between the retinal/chlorophyll a ratio and salinity. Retinal-based photoheterotrophy is particularly important for prokaryotic survival in hypersaline environments, surpassing the sunlight energy captured by photosynthesis, and being more relevant as salinity increases. This fact has implications for understanding the survival of microorganisms in extreme conditions and the energy dynamics in solar salter ponds.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Author(s). Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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