TMT-based quantitative proteomic analysis of IBDV-infected CEF cells reveals a favorable role of chicken IRF10 in IBDV replication via suppressing type-I interferon expression.

Autor:	Zhang S; National Key Laboratory of Veterinary Public Health Security; Animal Epidemiology of the Ministry of Agriculture; College of Veterinary Medicine, China Agricultural University, Beijing 100193, China., Li J; China Institute of Veterinary Drug Control., Gao H; National Key Laboratory of Veterinary Public Health Security; Animal Epidemiology of the Ministry of Agriculture; College of Veterinary Medicine, China Agricultural University, Beijing 100193, China., Wang Y; National Key Laboratory of Veterinary Public Health Security; Animal Epidemiology of the Ministry of Agriculture; College of Veterinary Medicine, China Agricultural University, Beijing 100193, China., Cao H; National Key Laboratory of Veterinary Public Health Security; Animal Epidemiology of the Ministry of Agriculture; College of Veterinary Medicine, China Agricultural University, Beijing 100193, China., Li X; National Key Laboratory of Veterinary Public Health Security; Animal Epidemiology of the Ministry of Agriculture; College of Veterinary Medicine, China Agricultural University, Beijing 100193, China., Gao L; National Key Laboratory of Veterinary Public Health Security; Animal Epidemiology of the Ministry of Agriculture; College of Veterinary Medicine, China Agricultural University, Beijing 100193, China., Zheng SJ; National Key Laboratory of Veterinary Public Health Security; Animal Epidemiology of the Ministry of Agriculture; College of Veterinary Medicine, China Agricultural University, Beijing 100193, China. Electronic address: sjzheng@cau.edu.cn.
Jazyk:	angličtina
Zdroj:	Poultry science [Poult Sci] 2024 Dec; Vol. 103 (12), pp. 104421. Date of Electronic Publication: 2024 Oct 13.
DOI:	10.1016/j.psj.2024.104421
Abstrakt:	Infectious bursal disease (IBD) is an acute, highly contagious disease caused by infectious bursal disease virus (IBDV), causing huge economic losses to the poultry industry worldwide. Currently, the emerging variant strains of IBDV and new recombinants in the field are circulating in many countries and poses severe threats to the development of poultry industry. Elucidation of the pathogenesis of IBDV infection will be of great help to the development of vaccines for control of IBDV infection. In this study, liquid chromatography tandem-mass spectrometry (LC-MS/MS) combined with tandem mass tag (TMT) labeling was performed to determine the expressions of nucleus proteins in IBDV-infected chicken embryonic fibroblast (CEF) cells 24 h post-infection (hpi). Our data show that a total of 236 nucleus proteins were differentially expressed in IBDV-infected cells vs mock-infected controls, and that among those proteins, 171 were significantly upregulated while 65 downregulated. Bioinformatics analysis reveals that the differentially expressed proteins (DEPs) were mainly involved in immune response, DNA replication, mismatch repair, and RIG-I-like receptor (RLR) signaling. Consistently, the expression of ten selected upregulated genes (IRF10, IRF7, IRF1, STAT1, ATF3, GTF3A, CSRP3, RARB, BASP1, and NF-κB1) markedly increased as examined by quantitative real-time PCR (qRT-PCR). Furthermore, the expression of IRF10 was upregulated both in the cytoplasm and nucleus of DF-1 cells as examined by Western Blot. Moreover, knockdown of IRF10 remarkably inhibited IBDV replication via promoting IFN-I response, and overexpression of IRF10 significantly suppressed type I interferon and ISGs expression in both mock and IBDV-infected cells, suggesting that IRF10 serve as a negative regulator for host antiviral response. These results provide clues to further investigation into host-IBDV interactions and the underlying mechanisms of IBDV infection. Competing Interests: Declaration of competing interest We declare there are no conflicts of interest. The founding sponsors had no role in the design of the study, in the collection, analyses, or interpretation of data, in the writing of the manuscript, and in the decision to publish the results. (Copyright © 2024. Published by Elsevier Inc.)
Databáze:	MEDLINE
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