The simultaneous miR-155-5p overexpression and miR-223-3p inhibition can activate pEMT in oral squamous cell carcinoma.

Autor:	Zhou R; Xiamen Medical College, Department of Stomotology, Xiamen 361000, China.; Fujian College Engineering Research Center for Dental Biomaterials, Xiamen 361000, China., Chen Z; Xiamen Medical College, Department of Stomotology, Xiamen 361000, China.; Fujian College Engineering Research Center for Dental Biomaterials, Xiamen 361000, China., Cai Y; Xiamen Medical College, Department of Stomotology, Xiamen 361000, China.; Fujian College Engineering Research Center for Dental Biomaterials, Xiamen 361000, China., Zhang H; Xiamen Medical College, Department of Stomotology, Xiamen 361000, China.; Fujian College Engineering Research Center for Dental Biomaterials, Xiamen 361000, China., Mao S; Xiamen Medical College, Department of Stomotology, Xiamen 361000, China., Zhuang Y; Xiamen Medical College, Department of Stomotology, Xiamen 361000, China., Zheng J; Xiamen Medical College, Department of Stomotology, Xiamen 361000, China.
Jazyk:	angličtina
Zdroj:	Journal of applied oral science : revista FOB [J Appl Oral Sci] 2024 Oct 21; Vol. 32, pp. e20240215. Date of Electronic Publication: 2024 Oct 21 (Print Publication: 2024).
DOI:	10.1590/1678-7757-2024-0215
Abstrakt:	Objective: This study aims to explore the effects of miR-223-3p and miR-155-5p on epithelial-mesenchymal transition (EMT) and migration in oral squamous cell carcinoma (OSCC). Methodology: EMT markers (E-cadherin, N-cadherin, P120 catenin (P120ctn), and vimentin) expression was determined by qRT-PCR and western blot analysis in SCC-9 cells which overexpress miR-155-5p and/or not express miR-223-3p. Scratch assays and Transwell migration assays were conducted to evaluate cell migration ability. Results: When miR-223-3p was inhibited in OSCC cells, P120ctn and E-cadherin mRNA levels were dramatically downregulated (P<0.05), while N-cadherin levels were significantly upregulated, and the migration ability of OSCC cells increased. The overexpression of miR-155-5p in OSCC cells upregulated miR-223-3p significantly (34-fold) compared to the control group. It also led to significant downregulation of the mRNA of P120ctn and E-cadherin and significant upregulation of the mRNA of N-cadherin and Vimentin (P<0.05). Meanwhile, the migratory ability of OSCC cells significantly increased. When miR-155-5p was overexpressed while miR-223-3p was inhibited, the highest expression of E-cadherin and P120ctn mRNA and the lowest expression of N-cadherin(P<0.05) was observed. Simultaneously, tumor cell migration was significantly facilitated. Conclusion: miR-223-3p inhibits the migration of OSCC cells, while miR-155-5p can elevate the miR-223-3p mRNA expression. The simultaneous miR-155-5p overexpression and miR-223-3p inhibition can activate pEMT, increasing OSCC migration in vitro. This provides a novel approach and potential target for the effective treatment of OSCC.
Databáze:	MEDLINE
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