| Autor: |
Sati L; Department of Histology and Embryology, Akdeniz University School of Medicine, 07070 Antalya, Turkey., Varela L; Department of Comparative Medicine, Yale University School of Medicine, New Haven, CT 06520, USA.; Laboratory of Glia-Neuron Interactions in the Control of Hunger, Achucarro Basque Center for Neuroscience, 48940 Leioa, Vizcaya, Spain.; IKERBASQUE, Basque Foundation for Science, 48009 Bilbao, Vizcaya, Spain., Horvath TL; Department of Comparative Medicine, Yale University School of Medicine, New Haven, CT 06520, USA.; Laboratory of Glia-Neuron Interactions in the Control of Hunger, Achucarro Basque Center for Neuroscience, 48940 Leioa, Vizcaya, Spain.; IKERBASQUE, Basque Foundation for Science, 48009 Bilbao, Vizcaya, Spain., McGrath J; Departments of Comparative Medicine and Genetics, Yale University School of Medicine, New Haven, CT 06520, USA. |
| Abstrakt: |
Interspecies hybrids have nuclear contributions from two species but oocyte cytoplasm from only one. Species discordance may lead to altered nuclear reprogramming of the foreign paternal genome. We reasoned that initial reprogramming in same species cytoplasm plus creation of hybrids with zygote cytoplasm from both species, which we describe here, might enhance nuclear reprogramming and promote hybrid development. We report in Mus species that (i) mammalian nuclear/cytoplasmic hybrids can be created, (ii) they allow development and viability of a previously missing and uncharacterized hybrid class, (iii) different oocyte cytoplasm environments lead to different phenotypes of same nuclear hybrid genotype, and (iv) the novel hybrids exhibit sex ratio distortion with the absence of female progeny and represent a mammalian exception to Haldane's rule. Our results emphasize that interspecies hybrid phenotypes are not only the result of nuclear gene epistatic interactions but also cytonuclear interactions and that the latter have major impacts on fetal and placental growth and development. |
