3D Neurovascular Unit Tissue Model to Assess Responses to Traumatic Brain Injury.

Autor:	Power L; Graduate School of Biomedical Sciences, Tufts University, Boston, Massachusetts, USA.; Department of Biomedical Engineering, Tufts University, Medford, Massachusetts, USA., Shuhmaher R; Faculty of Biomedical Engineering, Technion - Israel Institute of Technology, Haifa, Israel., Houtz P; Department of Biomedical Engineering, Tufts University, Medford, Massachusetts, USA., Chen J; Department of Biomedical Engineering, Tufts University, Medford, Massachusetts, USA., Rudolph S; Department of Biomedical Engineering, Tufts University, Medford, Massachusetts, USA., Yuen J; Department of Biomedical Engineering, Tufts University, Medford, Massachusetts, USA., Machour M; Faculty of Biomedical Engineering, Technion - Israel Institute of Technology, Haifa, Israel., Levy E; Department of Pediatrics, Massachusetts General Hospital, Boston, Massachusetts, USA., Wu L; Department of Pediatrics, Massachusetts General Hospital, Boston, Massachusetts, USA., Levenberg S; Faculty of Biomedical Engineering, Technion - Israel Institute of Technology, Haifa, Israel., Whalen M; Department of Pediatrics, Massachusetts General Hospital, Boston, Massachusetts, USA., Chen Y; Department of Biomedical Engineering, Tufts University, Medford, Massachusetts, USA., Kaplan DL; Department of Biomedical Engineering, Tufts University, Medford, Massachusetts, USA.
Jazyk:	angličtina
Zdroj:	Journal of biomedical materials research. Part A [J Biomed Mater Res A] 2025 Jan; Vol. 113 (1), pp. e37816. Date of Electronic Publication: 2024 Oct 23.
DOI:	10.1002/jbm.a.37816
Abstrakt:	The neurovascular unit (NVU) is a critical interface in the central nervous system that links vascular interactions with glial and neural tissue. Disruption of the NVU has been linked to the onset and progression of neurodegenerative diseases. Despite its significance the NVU remains challenging to study in a physiologically relevant manner. Here, a 3D cell triculture model of the NVU is developed that incorporates human primary brain microvascular endothelial cells, astrocytes, and pericytes into a tissue system that can be sustained in vitro for several weeks. This tissue model helps recapitulate the complexity of the NVU and can be used to interrogate the mechanisms of disease and cell-cell interactions. The NVU tissue model displays elevated cell death and inflammatory responses following mechanical damage, to emulate traumatic brain injury (TBI) under controlled laboratory conditions, including lactate dehydrogenase (LDH) release, elevated inflammatory markers TNF-α and monocyte chemoattractant cytokines MCP-2 and MCP-3 and reduced expression of the tight junction marker ZO-1. This 3D tissue model serves as a tool for deciphering mechanisms of TBIs and immune responses associated with the NVU. (© 2024 Wiley Periodicals LLC.)
Databáze:	MEDLINE
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