Salt-Responsive Gut Microbiota Induces Sex-Specific Blood Pressure Changes.

Autor:	Bardhan P; Department of Physiology and Pharmacology, Microbiome Consortium, Center for Hypertension and Precision Medicine, University of Toledo College of Medicine and Life Sciences, OH (P.B., X.M., N.K.L., B.M., R.T., S.A., I.M., H.H., T.A.J., R.R.A., J.K., B.J., T.Y.)., Mei X; Department of Physiology and Pharmacology, Microbiome Consortium, Center for Hypertension and Precision Medicine, University of Toledo College of Medicine and Life Sciences, OH (P.B., X.M., N.K.L., B.M., R.T., S.A., I.M., H.H., T.A.J., R.R.A., J.K., B.J., T.Y.).; Now with Department of Pharmacy, North Sichuan Medical College, Nanchong, China (X.M.)., Lai NK; Department of Physiology and Pharmacology, Microbiome Consortium, Center for Hypertension and Precision Medicine, University of Toledo College of Medicine and Life Sciences, OH (P.B., X.M., N.K.L., B.M., R.T., S.A., I.M., H.H., T.A.J., R.R.A., J.K., B.J., T.Y.)., Mell B; Department of Physiology and Pharmacology, Microbiome Consortium, Center for Hypertension and Precision Medicine, University of Toledo College of Medicine and Life Sciences, OH (P.B., X.M., N.K.L., B.M., R.T., S.A., I.M., H.H., T.A.J., R.R.A., J.K., B.J., T.Y.)., Tummala R; Department of Physiology and Pharmacology, Microbiome Consortium, Center for Hypertension and Precision Medicine, University of Toledo College of Medicine and Life Sciences, OH (P.B., X.M., N.K.L., B.M., R.T., S.A., I.M., H.H., T.A.J., R.R.A., J.K., B.J., T.Y.)., Aryal S; Department of Physiology and Pharmacology, Microbiome Consortium, Center for Hypertension and Precision Medicine, University of Toledo College of Medicine and Life Sciences, OH (P.B., X.M., N.K.L., B.M., R.T., S.A., I.M., H.H., T.A.J., R.R.A., J.K., B.J., T.Y.)., Manandhar I; Department of Physiology and Pharmacology, Microbiome Consortium, Center for Hypertension and Precision Medicine, University of Toledo College of Medicine and Life Sciences, OH (P.B., X.M., N.K.L., B.M., R.T., S.A., I.M., H.H., T.A.J., R.R.A., J.K., B.J., T.Y.)., Hwang H; Department of Physiology and Pharmacology, Microbiome Consortium, Center for Hypertension and Precision Medicine, University of Toledo College of Medicine and Life Sciences, OH (P.B., X.M., N.K.L., B.M., R.T., S.A., I.M., H.H., T.A.J., R.R.A., J.K., B.J., T.Y.)., Jhuma TA; Department of Physiology and Pharmacology, Microbiome Consortium, Center for Hypertension and Precision Medicine, University of Toledo College of Medicine and Life Sciences, OH (P.B., X.M., N.K.L., B.M., R.T., S.A., I.M., H.H., T.A.J., R.R.A., J.K., B.J., T.Y.)., Atluri RR; Department of Physiology and Pharmacology, Microbiome Consortium, Center for Hypertension and Precision Medicine, University of Toledo College of Medicine and Life Sciences, OH (P.B., X.M., N.K.L., B.M., R.T., S.A., I.M., H.H., T.A.J., R.R.A., J.K., B.J., T.Y.)., Kyoung J; Department of Physiology and Pharmacology, Microbiome Consortium, Center for Hypertension and Precision Medicine, University of Toledo College of Medicine and Life Sciences, OH (P.B., X.M., N.K.L., B.M., R.T., S.A., I.M., H.H., T.A.J., R.R.A., J.K., B.J., T.Y.)., Li Y; Department of Physiology and Pathophysiology, Xi'an Jiaotong University School of Basic Medical Sciences, China (Y.L., H.-B.L.)., Joe B; Department of Physiology and Pharmacology, Microbiome Consortium, Center for Hypertension and Precision Medicine, University of Toledo College of Medicine and Life Sciences, OH (P.B., X.M., N.K.L., B.M., R.T., S.A., I.M., H.H., T.A.J., R.R.A., J.K., B.J., T.Y.)., Li HB; Department of Physiology and Pathophysiology, Xi'an Jiaotong University School of Basic Medical Sciences, China (Y.L., H.-B.L.)., Yang T; Department of Physiology and Pharmacology, Microbiome Consortium, Center for Hypertension and Precision Medicine, University of Toledo College of Medicine and Life Sciences, OH (P.B., X.M., N.K.L., B.M., R.T., S.A., I.M., H.H., T.A.J., R.R.A., J.K., B.J., T.Y.).
Jazyk:	angličtina
Zdroj:	Circulation research [Circ Res] 2024 Dec 06; Vol. 135 (12), pp. 1122-1137. Date of Electronic Publication: 2024 Oct 23.
DOI:	10.1161/CIRCRESAHA.124.325056
Abstrakt:	Background: Tryptophan metabolism is important in blood pressure regulation. The tryptophan-indole pathway is exclusively mediated by the gut microbiota. ACE2 (angiotensin-converting enzyme 2) participates in tryptophan absorption, and a lack of ACE2 leads to changes in the gut microbiota. The gut microbiota has been recognized as a regulator of blood pressure. Furthermore, there is ample evidence for sex differences in the gut microbiota. However, it is unclear whether such sex differences impact blood pressure differentially through the tryptophan-indole pathway. Methods: To study the sex-specific mechanisms of gut microbiota-mediated tryptophan-indole pathway in hypertension, we generated a novel rat model with Clustered Regularly Interspaced Short Palindromic Repeats/Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats-associated protein 9)-targeted deletion of Ace2 in the Dahl salt-sensitive rat. Cecal microbiota transfers from donors of both sexes to female S recipients were performed. Also, Dahl salt-sensitive rats of both sexes were orally gavaged with indole to investigate blood pressure response. Results: The female gut microbiota and its tryptophan-indole pathway exhibited greater buffering capacity when exposed to tryptophan, due to Ace2 deficiency, and salt. In contrast, the male gut microbiota and its tryptophan-indole pathway were more vulnerable. Female rats with male cecal microbiota responded to salt with a higher blood pressure increase compared with those with female cecal microbiota. Indole, a tryptophan-derived metabolite produced by gut bacteria, increased blood pressure in male but not in female rats. Moreover, salt altered host-mediated tryptophan metabolism, characterized by reduced serum serotonin of both sexes and higher levels of kynurenine derivatives in the females. Conclusions: We uncovered a novel sex-specific mechanism in the gut microbiota-mediated tryptophan-indole pathway in blood pressure regulation. Salt tipped the tryptophan metabolism between the host and gut microbiota in a sex-dependent manner. Our study provides evidence for a novel concept that gut microbiota and its metabolism play sex-specific roles in the development of salt-sensitive hypertension. Competing Interests: None.
Databáze:	MEDLINE
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