| Autor: |
Park H; Department of Chemical Engineering, Kwangwoon University, 20 Gwangwoon-Ro, Nowon-Gu, Seoul 01897, Republic of Korea. andy9760@kw.ac.k., Kim SW; Department of Chemistry, Kwangwoon University, 20 Gwangwoon-Ro, Nowon-Gu, Seoul 01897, Republic of Korea. tom2827@nate.com., Lee S; Department of Chemical Engineering, Kwangwoon University, 20 Gwangwoon-Ro, Nowon-Gu, Seoul 01897, Republic of Korea. andy9760@kw.ac.k., An J; Department of Chemical Engineering, Kwangwoon University, 20 Gwangwoon-Ro, Nowon-Gu, Seoul 01897, Republic of Korea. andy9760@kw.ac.k., Jung S; Department of Chemical Engineering, Kwangwoon University, 20 Gwangwoon-Ro, Nowon-Gu, Seoul 01897, Republic of Korea. andy9760@kw.ac.k., Lee M; Department of Electronics Convergence Engineering, Kwangwoon University, 20 Gwangwoon-Ro, Nowon-Gu, Seoul 01897, Republic of Korea. ammylee12345@naver.com., Kim J; Department of Electronics Convergence Engineering, Kwangwoon University, 20 Gwangwoon-Ro, Nowon-Gu, Seoul 01897, Republic of Korea. ammylee12345@naver.com., Kwon D; Protist Research Division, Biological Resources Research Department, Nakdonggang National Institute of Biological Resources (NNIBR), 137, Donam 2-gil, Sangju-si, Gyeongsangbuk-do 37242, Republic of Korea. kdyrevive@nnibr.re.kr., Jang H; Department of Chemistry, Kwangwoon University, 20 Gwangwoon-Ro, Nowon-Gu, Seoul 01897, Republic of Korea. tom2827@nate.com., Lee T; Department of Chemical Engineering, Kwangwoon University, 20 Gwangwoon-Ro, Nowon-Gu, Seoul 01897, Republic of Korea. andy9760@kw.ac.k. |
| Abstrakt: |
Cyanobacteria play an essential role in nutrient cycling in aquatic ecosystems. However, certain species adversely affect the environment and human health by causing harmful cyanobacterial algal blooms (cyanoHABs) and producing cyanotoxins. To address this issue, continuous cyanoHAB monitoring has been considered; however, a gold standard has not yet been established. In this study, we aimed to develop a dual DNA-targeting capacitive-type biosensor for rapid field-ready monitoring of Raphidiopsis raciborskii , a causative species of cyanoHAB. To enhance the sensing signal, a plate-like Au-BiOCl nanocomposite was synthesized using a spontaneous carbonation process without additional additives. The alternating-current electrothermal flow (ACEF) technique was applied to enable rapid DNA and probe binding within 10 min. The limits of detection (LODs) for R. raciborskii RubisCO large subunit ( rbcL ) and RNA polymerase beta subunit ( rpoB ) genes diluted in deionized (DI) water were 4.89 × 10 -17 and 3.89 × 10 -17 M, respectively. Furthermore, the LODs of R. raciborskii rbcl and rpoB diluted in freshwater containing HAB were 2.55 × 10 -16 and 3.84 × 10 -16 M, respectively, demonstrating the field-ready applicability of the device. The fabricated cyanobacterial DNA-sensing platform enabled powerful species-specific detection using a small sample volume and low target concentration without a nucleic acid amplification step, dramatically reducing the detection time. This study has considerable implications for detecting HABs, early warning systems, and species-specific environmental monitoring technology. |
