Characterization of cellular and molecular immune components of the painted white sea urchin Lytechinus pictus in response to bacterial infection.

Autor: Nesbit KT; Department of Biology, San Diego State University, San Diego, CA, USA., Hargadon AC; Scripps Institution of Oceanography, University of California San Diego, San Diego, CA, USA., Renaudin GD; Scripps Institution of Oceanography, University of California San Diego, San Diego, CA, USA., Kraieski ND; Department of Biological Sciences, Auburn University, Auburn, AL, USA., Buckley KM; Department of Biological Sciences, Auburn University, Auburn, AL, USA., Darin E; Department of Biology, San Diego State University, San Diego, CA, USA., Lee Y; Scripps Institution of Oceanography, University of California San Diego, San Diego, CA, USA., Hamdoun A; Scripps Institution of Oceanography, University of California San Diego, San Diego, CA, USA., Schrankel CS; Department of Biology, San Diego State University, San Diego, CA, USA.
Jazyk: angličtina
Zdroj: Immunology and cell biology [Immunol Cell Biol] 2024 Oct 22. Date of Electronic Publication: 2024 Oct 22.
DOI: 10.1111/imcb.12828
Abstrakt: Sea urchins are basal deuterostomes that share key molecular components of innate immunity with vertebrates. They are a powerful model for the study of innate immune system evolution and function, especially during early development. Here we characterize the morphology and associated molecular markers of larval immune cell types in a newly developed model sea urchin, Lytechinus pictus. We then challenge larvae through infection with an established pathogenic Vibrio and characterize phenotypic and molecular responses. We contrast these to the previously described immune responses of the purple sea urchin Strongylocentrotus purpuratus. The results revealed shared cellular morphologies and homologs of known pigment cell immunocyte markers (PKS, srcr142) but a striking absence of subsets of perforin-like macpf genes in blastocoelar cell immunocytes. We also identified novel patterning of cells expressing a scavenger receptor cysteine rich (SRCR) gene in the coelomic pouches of the larva (the embryonic stem cell niche). The SRCR signal becomes further enriched in both pouches in response to bacterial infection. Collectively, these results provide a foundation for the study of immune responses in L. pictus. The characterization of the larval immune system of this rapidly developing and genetically enabled sea urchin species will facilitate more sophisticated studies of innate immunity and the crosstalk between the immune system and development.
(© 2024 The Author(s). Immunology & Cell Biology published by John Wiley & Sons Australia, Ltd on behalf of the Australian and New Zealand Society for Immunology, Inc.)
Databáze: MEDLINE
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