A Proteogenomic Approach for the Identification of Virulence Factors in Leishmania Parasites.
| Autor: | Munjal NS; Institute of Bioinformatics, Bangalore, India., Dey G; Institute of Bioinformatics, Bangalore, India., Parthasarathi KTS; Institute of Bioinformatics, Bangalore, India.; Manipal Academy of Higher Education, Manipal, Karnataka, India., Chauhan K; School of Applied Sciences and Technology, Gujarat Technological University, Ahmedabad, India., Pai K; Department of Zoology, Savitribai Phule Pune University, Pune, India., Patole MS; National Centre for Cell Science, Pune, India., Pawar H; Biomedical and Life Sciences Division, Lancaster University, Lancaster, UK., Sharma J; Institute of Bioinformatics, Bangalore, India. jyoti@ibioinformatics.org.; Manipal Academy of Higher Education, Manipal, Karnataka, India. jyoti@ibioinformatics.org. |
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| Jazyk: | angličtina |
| Zdroj: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2025; Vol. 2859, pp. 279-296. |
| DOI: | 10.1007/978-1-0716-4152-1_16 |
| Abstrakt: | Identifying new genes involved in virulence and drug resistance may hold the key to a better understanding of parasitic diseases. The proteogenomic profiling of various Leishmania species, the causative agents of leishmaniasis, has identified several novel genes, N- and C-terminal extensions of proteins, and corrections of existing gene models. Various virulence factors (VFs) responsible for leishmaniasis have been previously annotated through a proteogenomic approach, including the C-terminal extension of heat shock protein 70 (HSP70). Furthermore, the diversity of VFs across Leishmania donovani, L. infantum, L. major, and L. mexicana was determined using phylogenetic analysis. Moreover, protein-protein interaction networks (PPINs) of VFs with HSPs aid in making significant biological interpretations. Overall, an integrated omics approach involving proteogenomics was used to identify and study the relationship among VFs with other interacting proteins, including HSPs. This chapter provides a step-by-step guide to the identification of new genes in Leishmania using a proteogenomic approach and their functional assignment using a bioinformatics-based approach. (© 2025. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.) |
| Databáze: | MEDLINE |
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