Evaluation of a targeted anti-α v β 3 integrin near-infrared fluorescent dye for fluorescence-guided resection of naturally occurring soft tissue sarcomas in dogs.
| Autor: | Beer P; Clinic for Small Animal Surgery, Vetsuisse Faculty, University Animal Hospital, University of Zurich, Zurich, Switzerland. pbeer@vetclinics.uzh.ch., Grest P; Institute of Veterinary Pathology, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland., Krudewig C; Institute of Veterinary Pathology, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland., Staudinger C; Clinic for Diagnostic Imaging, University Animal Hospital, Vetsuisse Faculty Zurich, University Zurich, Zurich, Switzerland., Ohlerth S; Clinic for Diagnostic Imaging, University Animal Hospital, Vetsuisse Faculty Zurich, University Zurich, Zurich, Switzerland., Rohrer Bley C; Division of Radiation Oncology, University Animal Hospital, Vetsuisse Faculty Zurich, University Zurich, Zurich, Switzerland., Jarosch A; Department of Pathology, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Berlin, Germany., Ech-Cherif H; Institute of Veterinary Pharmacology and Toxicology, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland., Markkanen E; Institute of Veterinary Pharmacology and Toxicology, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland., Park B; Clinic for Small Animal Surgery, Vetsuisse Faculty, University Animal Hospital, University of Zurich, Zurich, Switzerland., Nolff MC; Clinic for Small Animal Surgery, Vetsuisse Faculty, University Animal Hospital, University of Zurich, Zurich, Switzerland. |
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| Jazyk: | angličtina |
| Zdroj: | European journal of nuclear medicine and molecular imaging [Eur J Nucl Med Mol Imaging] 2024 Oct 22. Date of Electronic Publication: 2024 Oct 22. |
| DOI: | 10.1007/s00259-024-06953-x |
| Abstrakt: | Purpose: Complete resection is a key prognostic factor for survival in patients with soft tissue sarcoma (STS), in humas and companion animals alike. Fluorescence-guided surgery could improve resection accuracy. As dogs are frequently affected by STS, they serve as a model to test an anti-α Methods: Twenty dogs with STS were randomly allocated to either receive Angiostamp™ (NIRF group) or physiologic saline (control group) preoperatively. The researchers were blinded for treatment, and resections were adapted based on the NIRF-signal, if needed. Margin status was histologically determined at the 1 and 3 cm margin. The tumor-to-background ratio was measured in native tissue biopsies and formalin-fixed tissue. The fluorescent area was compared to the corresponding tumor areas as confirmed by histology using the Dice coefficient. Target expression was quantified by immunohistochemistry and correlated to NIRF-signal ratios. Results: A fluorescent signal was detected in all 10 tumors of the NIRF group, with a tumor-to-background ratio of 7.4 ± 5.8 in native biopsies and 13.5 ± 10.9 in formalin-fixed tissue. In the NIRF group, resection margins were adapted in 5/10 cases, leading to complete resection and preventing R1 in four of these cases. In the NIRF and control group 9/10 and 8/10 resections were R0, with one local recurrence in each group and one sarcoma-related death in the NIRF group. The NIRF-signal correlated with the histologically confirmed tumor area (Dice coefficient 0.75 ± 0.17). Target expression was higher in tumor compared to peritumoral tissue (p < 0.0003) and showed a moderate correlation with the NIRF-signal (r = 0.6516, p < 0.0001). Conclusion: Fluorescence-guided surgery using Angiostamp™ can pinpoint residual disease in the tumor bed and contributes to an improved resection accuracy in canine STS. (© 2024. The Author(s).) |
| Databáze: | MEDLINE |
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