The MRGPRX2-substance P pathway regulates mast cell migration.

Autor:	West PW; Lydia Becker Institute of Immunology and Inflammation, School of Biological Sciences, Faculty of Biology, Medicine and Health, University of Manchester, Manchester Academic Health Science Centre, Manchester, UK., Chéret J; Dr. Phillip Frost Department of Dermatology and Cutaneous Surgery, University of Miami Miller School of Medicine, Miami, FL, USA.; CUTANEON- Skin & Hair Innovation, Hamburg, Berlin, Germany., Bahri R; Lydia Becker Institute of Immunology and Inflammation, School of Biological Sciences, Faculty of Biology, Medicine and Health, University of Manchester, Manchester Academic Health Science Centre, Manchester, UK., Kiss O; Lydia Becker Institute of Immunology and Inflammation, School of Biological Sciences, Faculty of Biology, Medicine and Health, University of Manchester, Manchester Academic Health Science Centre, Manchester, UK., Wu Z; GSK, 1250 South Collegeville Road, Collegeville, PA 19426, USA., Macphee CH; GSK, 1250 South Collegeville Road, Collegeville, PA 19426, USA., Bulfone-Paus S; Lydia Becker Institute of Immunology and Inflammation, School of Biological Sciences, Faculty of Biology, Medicine and Health, University of Manchester, Manchester Academic Health Science Centre, Manchester, UK.
Jazyk:	angličtina
Zdroj:	IScience [iScience] 2024 Sep 16; Vol. 27 (10), pp. 110984. Date of Electronic Publication: 2024 Sep 16 (Print Publication: 2024).
DOI:	10.1016/j.isci.2024.110984
Abstrakt:	Mast cells (MCs) are tissue-resident immune cells known to degranulate in response to FcεRI crosslinking or MRGPRX2 engagement. MCs are found close to nerves, but the mechanisms that regulate this privileged localization remain unclear. Here, we investigated MRGPRX2 expression patterns and specific activities in MCs. We show that MRGPRX2 expression is heterogeneous in human MC (hMC) progenitors and mature MCs. Substance P (SP) is a rapid and specific activator of MRGPRX2, and long-term supplementation of MCs with SP expands MRGPRX2-expressing cells. While high concentrations of SP induce rapid MC degranulation, low concentrations prompt immature MC chemotaxis. Lastly, we demonstrate that in inflammatory skin conditions like psoriasis, the number of MRGPRX2 + MCs is increased, and during in vitro skin reinnervation, MRGPRX2 + MCs preferentially reside in proximity to and migrate toward SP + nerve fibers (NFs). This indicates that SP-MRGPRX2 signaling defines MC positioning and relocation within tissues and promotes immune cell-NF communication. Competing Interests: Z.W. and C.H.M. are employees at GSK Collegeville. (© 2024 The Author(s).)
Databáze:	MEDLINE
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