Biofilm Demolition by [Au III (N N)Cl(NHC)][PF 6 ] 2 Complexes Fastened with Bipyridine and Phenanthroline Ligands; Potent Antibacterial Agents Targeting Membrane Lipid.

Autor: Sahu P; Department of Chemistry, Utkal University, Vani Vihar, Bhubaneswar, Odisha, 751004, India., Chakraborty S; Department of Bioscience and Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur, West Bengal, 721302, India., Isab AA; Department of Chemistry, King Fahd University of Petroleum and Minerals, Dhahran, 31261, Saudi Arabia., Mandal SM; Department of Bioscience and Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur, West Bengal, 721302, India., Dinda J; Department of Chemistry, Utkal University, Vani Vihar, Bhubaneswar, Odisha, 751004, India.
Jazyk: angličtina
Zdroj: ChemPlusChem [Chempluschem] 2024 Oct 22, pp. e202400543. Date of Electronic Publication: 2024 Oct 22.
DOI: 10.1002/cplu.202400543
Abstrakt: The development of new antibacterial drugs is essential for staying ahead of evolving antibiotic resistant bacterial (ARB) threats, ensuring effective treatment options for bacterial infections, and protecting public health. Herein, we successfully designed and synthesized two novel gold(III)- NHC complexes, [Au(1)(bpy)Cl][PF 6 ] 2 (2) and [Au(1)(phen)Cl][PF 6 ] 2 (3) based on the proligand pyridyl[1,2-a]{2-pyridylimidazol}-3-ylidene hexafluorophosphate (1⋅HPF 6 ) [bpy=2,2'-bipyridine; phen=1,10-phenanthroline]. The synthesized complexes were characterized spectroscopically; their geometries and structural arrangements were confirmed by single crystal XRD analysis. Complexes 2 and 3 showed photoluminescence properties at room temperature and the time-resolved fluorescence decay confirmed the fluorescence lifetimes of 0.54 and 0.62 ns respectively; which were used to demonstrate their direct interaction with bacterial cells. Among the two complexes, complex 3 was found to be more potent against the bacterial strains (Staphylococcus aureus, Gram-positive and Pseudomonas aeruginosa, Gram-negative bacteria) with the MIC values of 8.91 μM and 17.82 μM respectively. Studies revealed the binding of the complexes with the fundamental phospholipids present in the cell membrane of bacteria, which was found to be the leading cause of bacterial cell death. Cytotoxicity was evaluated using an MTT assay on 293 T cell lines; emphasizing the potential therapeutic uses of the Au(III)-NHC complexes to control bacterial infections.
(© 2024 Wiley-VCH GmbH.)
Databáze: MEDLINE
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