Silver nanoparticles synthesized using aerial part of Achillea fragrantissima and evaluation of their bioactivities.

Autor: Alsayed MF; Department of Botany and Microbiology, College of Science, King Saud University, 11451, Riyadh, Saudi Arabia., Alodaini HA; Department of Botany and Microbiology, College of Science, King Saud University, 11451, Riyadh, Saudi Arabia., Aziz IM; Department of Botany and Microbiology, College of Science, King Saud University, 11451, Riyadh, Saudi Arabia. iaziz@ksu.edu.sa., Alshalan RM; Department of Botany and Microbiology, College of Science, King Saud University, 11451, Riyadh, Saudi Arabia., Rizwana H; Department of Botany and Microbiology, College of Science, King Saud University, 11451, Riyadh, Saudi Arabia., Alkhelaiwi F; Department of Botany and Microbiology, College of Science, King Saud University, 11451, Riyadh, Saudi Arabia., ALSaigh SM; Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, P.O. Box 10219, 11433, Riyadh, Saudi Arabia., Alkubaisi NA; Department of Botany and Microbiology, College of Science, King Saud University, 11451, Riyadh, Saudi Arabia.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2024 Oct 21; Vol. 14 (1), pp. 24703. Date of Electronic Publication: 2024 Oct 21.
DOI: 10.1038/s41598-024-75558-z
Abstrakt: Achillea fragrantissima (A. fragrantissima), a desert plant, is used internally in Arabian traditional medicine to treat inflammatory, spasmodic gastrointestinal disorders, and hepatobiliary diseases. The study focuses on the environmentally friendly production of silver nanoparticles (AgNPs) from the water-based aerial parts of the A. fragrantissima plant and their ability to kill bacteria and cells. Ultraviolet-visible (UV-Vis) spectroscopy, scanning electron microscopy (SEM) with energy dispersive X-ray (EDX) analysis, transmission electron microscopy (TEM), and Fourier transform infrared spectroscopy (FTIR) were used to describe the AgNPs. They were then tested for their ability to fight cancer and bacteria. A change in colour from yellow to brown and a surface plasmon resonance peak at 440 nm, seen with UV-Vis spectroscopy, showed that AgNPs had formed. In a Gas Chromatography-Mass Spectrometry (GC-MS) test of the aerial parts of A. fragrantissima, twenty bioactive components were found. These included isolongifolol and 3E,10Z-Oxacyclotrideca-3,10-diene-2,7-dione, methylbuta-1,3-dienyl)-7-oxabicyclo [4.1.0] heptan-3-ol. The extract exhibited high phenolic and flavonoid content (77.52 ± 1.46 mg GAE/g dry weight and 59 ± 2.17 mg QE/g dry weight, respectively). According to the IC 50 values of 17.2 ± 1.18 and 14 ± 2.43 µg/mL, the AgNPs had a lot of power to kill cancer cells from the MCF-7 and HepG2 lines. Some genes that cause cell death (caspase-3, 8, 9, and Bax) were turned on more in the treated cells compared to the control cells that had not been treated. These genes were Bcl-xL and Bcl-2. Additionally, substantial activity against both Gram-positive bacteria and Gram-negative bacteria was found by antibacterial screening. Overall, this study underscores A. fragrantissima's diverse biological activity and its potential in drug discovery and nanomedicine, promoting the development of natural antibacterial and anticancer therapies.
(© 2024. The Author(s).)
Databáze: MEDLINE
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