Bacillus endophytic strains control Fusarium wilt caused by Fusarium oxysporum f. sp. lycopersici in tomato cv. Perinha.

Autor: Viana GC; Universidade Federal Do Rio de Janeiro, UFRJ - Ilha Do Fundão - Avenida Carlos Chagas Filho, 373 - Cidade Universitária da Universidade Federal Do Rio de Janeiro, Rio de Janeiro, RJ, 21.941-902, Brazil.; Empresa Brasileira de Pesquisa Agropecuária - Centro Nacional de Pesquisa de Agrobiologia, BR 465, Km 7, S/N, Seropédica, RJ, 23.891-000, Brazil., Médici LO; Laboratório de Fisiologia Vegetal, Instituto de Biologia, Departamento de Fisiologia Vegetal, Universidade Federal Rural Do Rio de Janeiro, Seropédica, 23891-000, Brazil., Vidal MS; Empresa Brasileira de Pesquisa Agropecuária - Centro Nacional de Pesquisa de Agrobiologia, BR 465, Km 7, S/N, Seropédica, RJ, 23.891-000, Brazil., Baldani JI; Empresa Brasileira de Pesquisa Agropecuária - Centro Nacional de Pesquisa de Agrobiologia, BR 465, Km 7, S/N, Seropédica, RJ, 23.891-000, Brazil. ivo.baldani@embrapa.br.
Jazyk: angličtina
Zdroj: Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] [Braz J Microbiol] 2024 Oct 22. Date of Electronic Publication: 2024 Oct 22.
DOI: 10.1007/s42770-024-01539-z
Abstrakt: Fusarium wilt is one of main phytopathology attacking tomato (Solanum lycopersicum L.) plantations in Brazil. Plant rhizosphere and endophytic beneficial microorganism are well known as plant growth promoters and biocontrol agents. The present study aims to evaluate the potential of different Bacillus strains as biocontrol agent to Fusarium oxysporum f. sp. lycopersici Race 3 strains; and also as plant growth promoting bacteria on Solanum lycopersicum cv Perinha. Different in vitro and greenhouse experiments were carried out to evaluate the direct and indirect bacterial-fungus antagonism, and they inoculation effects on plant traits. In vitro direct, metabolites, and volatile antagonism analysis demonstrated that B. toyonensis BR 10491(FORT 02) presented a broad antagonism to all tested race 3 FOL strains while B. megaterium BR 10466 (FORT 12), B. aryabhattai BR 10494 (FORT 25), B. stratosphericus BR 10438 (FORT 29) and B. cereus BR 10493 (FORT 113.1) strains showed significant antagonistic activity for at least two applied methods. Greenhouse pot experiments demonstrated a significant BCA effect of FORT 113.1 and FORT 02 against FOL Race 3 Fus 1302 strain during different tomato development stages (seedling, vegetative, and reproductive). Bacillus cereus (FORT 113.1) showed significantly higher shoot and height fresh weight, Chlorophyll a and Chlorophyll b content, stomata conductance, water use efficiency, and also a lower xylem infection percentage during vegetative and reproductive stages. Antioxidant enzymatic components analysis demonstrated a synergic effect of Fusarium and Bacillus inoculation, leading to a higher superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX) activity. In conclusion, the results suggest that strain FORT113.1 could be considered as a good candidate for production of new biofungicide with high potential to augment the existing biocontrol strategies.
(© 2024. The Author(s) under exclusive licence to Sociedade Brasileira de Microbiologia.)
Databáze: MEDLINE
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