CENP-C-Mis12 complex establishes a regulatory loop through Aurora B for chromosome segregation.

Autor:	Kong W; https://ror.org/035t8zc32 Graduate School of Frontier Biosciences, Osaka University, Osaka, Japan., Hara M; https://ror.org/035t8zc32 Graduate School of Frontier Biosciences, Osaka University, Osaka, Japan m.hara.fbs@osaka-u.ac.jp., Tokunaga Y; https://ror.org/02120t614 Division of Experimental Animal Research, Cancer Genome Center, Chiba Cancer Center Research Institute, Chiba, Japan., Okumura K; https://ror.org/02120t614 Division of Experimental Animal Research, Cancer Genome Center, Chiba Cancer Center Research Institute, Chiba, Japan., Hirano Y; https://ror.org/035t8zc32 Graduate School of Frontier Biosciences, Osaka University, Osaka, Japan., Miao J; https://ror.org/035t8zc32 Graduate School of Frontier Biosciences, Osaka University, Osaka, Japan., Takenoshita Y; https://ror.org/035t8zc32 Graduate School of Frontier Biosciences, Osaka University, Osaka, Japan., Hashimoto M; https://ror.org/035t8zc32 Graduate School of Frontier Biosciences, Osaka University, Osaka, Japan.; Department of Cell Science, Institute of Biomedical Sciences, School of Medicine, Fukushima Medical University, Fukushima, Japan., Sasaki H; https://ror.org/035t8zc32 Graduate School of Frontier Biosciences, Osaka University, Osaka, Japan., Fujimori T; Division of Embryology, National Institute for Basic Biology, Okazaki, Japan.; Basic Biology Program, The Graduate University for Advanced Studies, Okazaki, Japan., Wakabayashi Y; https://ror.org/02120t614 Division of Experimental Animal Research, Cancer Genome Center, Chiba Cancer Center Research Institute, Chiba, Japan., Fukagawa T; https://ror.org/035t8zc32 Graduate School of Frontier Biosciences, Osaka University, Osaka, Japan fukagawa.tatsuo.fbs@osaka-u.ac.jp.
Jazyk:	angličtina
Zdroj:	Life science alliance [Life Sci Alliance] 2024 Oct 21; Vol. 8 (1). Date of Electronic Publication: 2024 Oct 21 (Print Publication: 2025).
DOI:	10.26508/lsa.202402927
Abstrakt:	Establishing the correct kinetochore-microtubule attachment is crucial for faithful chromosome segregation. The kinetochore has various regulatory mechanisms for establishing correct bipolar attachment. However, how the regulations are coupled is not fully understood. Here, we demonstrate a regulatory loop between the kinetochore protein CENP-C and Aurora B kinase, which is critical for the error correction of kinetochore-microtubule attachment. This regulatory loop is mediated through the binding of CENP-C to the outer kinetochore Mis12 complex (Mis12C). Although the Mis12C-binding region of CENP-C is dispensable for mouse development and proliferation in human RPE-1 cells, those cells lacking this region display increased mitotic defects. The CENP-C-Mis12C interaction facilitates the centromeric recruitment of Aurora B and the mitotic error correction in human cells. Given that Aurora B reinforces the CENP-C-Mis12C interaction, our findings reveal a positive regulatory loop between Aurora B recruitment and the CENP-C-Mis12C interaction, which ensures chromosome biorientation for accurate chromosome segregation. (© 2024 Kong et al.)
Databáze:	MEDLINE
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