Evaluation of chemical constituents in Norantea guianensis aubl. Extracts, embryotoxicity, and acetylcholinesterase inhibitory potential in Danio rerio.

Autor: Sousa do Nascimento Monteiro L; Graduate Program in Environment and Regional Development, Universidade Anhanguera -Uniderp. Rua Alexandre Herculano, 1400, Jardim Veraneio, 79037-280, Campo Grande, Mato Grosso do Sul, Brazil., Matias R; Graduate Program in Environment and Regional Development, Universidade Anhanguera -Uniderp. Rua Alexandre Herculano, 1400, Jardim Veraneio, 79037-280, Campo Grande, Mato Grosso do Sul, Brazil., Fernandes CE; Graduate Program in Animal Biology, Federal University of Mato Grosso do Sul. Av. Costa e Silva, Pioneiros, 79070-900, Campo Grande, Mato Grosso do Sul, Brazil., Otsubo Jaques JA; Programa Multicêntrico de Pós-Graduação em Bioquímica e Biologia Molecular (PBBqBM), Instituto de Biociências (INBIO), Universidade Federal de Mato Grosso do Sul (UFMS), Campo Grande, Mato Grosso do Sul, Brazil., Brito IL; Programa Multicêntrico de Pós-Graduação em Bioquímica e Biologia Molecular (PBBqBM), Instituto de Biociências (INBIO), Universidade Federal de Mato Grosso do Sul (UFMS), Campo Grande, Mato Grosso do Sul, Brazil., Morbeck de Oliveira AK; Graduate Program in Environment and Regional Development, Universidade Anhanguera -Uniderp. Rua Alexandre Herculano, 1400, Jardim Veraneio, 79037-280, Campo Grande, Mato Grosso do Sul, Brazil., Facco GG; Graduate Program in Environment and Regional Development, Universidade Anhanguera -Uniderp. Rua Alexandre Herculano, 1400, Jardim Veraneio, 79037-280, Campo Grande, Mato Grosso do Sul, Brazil., Gediel Rivero-Wendt CL; Graduate Program in Animal Biology, Federal University of Mato Grosso do Sul. Av. Costa e Silva, Pioneiros, 79070-900, Campo Grande, Mato Grosso do Sul, Brazil. Electronic address: leticiagediel@gmail.com.
Jazyk: angličtina
Zdroj: Toxicon : official journal of the International Society on Toxinology [Toxicon] 2024 Nov 28; Vol. 251, pp. 108132. Date of Electronic Publication: 2024 Oct 20.
DOI: 10.1016/j.toxicon.2024.108132
Abstrakt: Norantea guianensis Aubl., commonly referred to as the parrot flower or macaw-tail vine, is a plant species found in the Brazilian Amazon, Caatinga, and Cerrado biomes. It is used in folk medicine for its anxiolytic properties, as well as its ability to alleviate headaches and reduce fever. Nevertheless, despite its therapeutic benefits, the impact on embryonic development has yet to be fully explained. The objective of this study was to evaluate chemical constituents by HPLC-DAD, UV-visible and classical phytochemistry and the LC50 of ethanolic extracts from the stem and leaves of N. guianensis in Danio rerio after 96 h and to investigate their effect on developmental morphology. The findings were then related to both Acetylcholinesterase (AChE) activity and the plant's chemical composition. Zebrafish embryos were exposed to 0, 20, 40, 60, 80 and 100 mg/L concentrations of stem and leaves extracts. Phytochemical analysis revealed that the stem extract contained predominantly phenolic compounds, tannins, and anthraquinones, while the leaf extract contained alkaloids and flavonoids. The LC50 values for the stem and leaf extracts were 64.55 mg/L and 7.16 mg/L, respectively, being the stem extract was more toxic than the leaf extract. Induced malformations and alterations in the zebrafish development in different concentrations for both extract including pericardial edema, increased heart rate, spinal malformation and equilibrium disruption. Unlike to stem extract, the leaf extract delayed larval hatching and inhibited AChE activity. The findings indicate that the leaf extract possesses higher embryotoxicity and its use should be avoided during pregnancy.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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