A reliable strategy for establishment of an animal model of diabetic cardiomyopathy: Induction by a high-fat diet combined with single or multiple injections of low-dose streptozotocin.

Autor: Wen W; Department of Cardiology, Shunde Hospital, Southern Medical University (The First People's Hospital of Shunde), NO. 1 Jiazi Road, Lunjiao, Shunde District, Foshan City, Guangdong 528308, China; Medical Research Center, Shunde Hospital, Southern Medical University (The First People's Hospital of Shunde) NO. 1 Jiazi Road, Lunjiao, Shunde District, Foshan City, Guangdong 528308, China. Electronic address: starwain@smu.edu.cn., Cao Y; Department of Cardiology, Shunde Hospital, Southern Medical University (The First People's Hospital of Shunde), NO. 1 Jiazi Road, Lunjiao, Shunde District, Foshan City, Guangdong 528308, China; Medical Research Center, Shunde Hospital, Southern Medical University (The First People's Hospital of Shunde) NO. 1 Jiazi Road, Lunjiao, Shunde District, Foshan City, Guangdong 528308, China. Electronic address: 21920504@smu.edu.cn., Chen P; Department of Cardiology, Shunde Hospital, Southern Medical University (The First People's Hospital of Shunde), NO. 1 Jiazi Road, Lunjiao, Shunde District, Foshan City, Guangdong 528308, China; Medical Research Center, Shunde Hospital, Southern Medical University (The First People's Hospital of Shunde) NO. 1 Jiazi Road, Lunjiao, Shunde District, Foshan City, Guangdong 528308, China. Electronic address: cp22110263@smu.edu.cn., Li J; Department of Cardiology, Shunde Hospital, Southern Medical University (The First People's Hospital of Shunde), NO. 1 Jiazi Road, Lunjiao, Shunde District, Foshan City, Guangdong 528308, China; Medical Research Center, Shunde Hospital, Southern Medical University (The First People's Hospital of Shunde) NO. 1 Jiazi Road, Lunjiao, Shunde District, Foshan City, Guangdong 528308, China. Electronic address: l22210342@smu.edu.cn., Li W; Department of Cardiology, Shunde Hospital, Southern Medical University (The First People's Hospital of Shunde), NO. 1 Jiazi Road, Lunjiao, Shunde District, Foshan City, Guangdong 528308, China; Medical Research Center, Shunde Hospital, Southern Medical University (The First People's Hospital of Shunde) NO. 1 Jiazi Road, Lunjiao, Shunde District, Foshan City, Guangdong 528308, China. Electronic address: liweiwen9608@smu.edu.cn., Huang G; Department of Cardiology, Shunde Hospital, Southern Medical University (The First People's Hospital of Shunde), NO. 1 Jiazi Road, Lunjiao, Shunde District, Foshan City, Guangdong 528308, China; Medical Research Center, Shunde Hospital, Southern Medical University (The First People's Hospital of Shunde) NO. 1 Jiazi Road, Lunjiao, Shunde District, Foshan City, Guangdong 528308, China. Electronic address: hgl131926@smu.edu.cn., Zheng H; Department of Cardiology, Shunde Hospital, Southern Medical University (The First People's Hospital of Shunde), NO. 1 Jiazi Road, Lunjiao, Shunde District, Foshan City, Guangdong 528308, China; Medical Research Center, Shunde Hospital, Southern Medical University (The First People's Hospital of Shunde) NO. 1 Jiazi Road, Lunjiao, Shunde District, Foshan City, Guangdong 528308, China. Electronic address: zhenghaoxiao@smu.edu.cn., Zhu X; Department of Cardiology, Shunde Hospital, Southern Medical University (The First People's Hospital of Shunde), NO. 1 Jiazi Road, Lunjiao, Shunde District, Foshan City, Guangdong 528308, China; Medical Research Center, Shunde Hospital, Southern Medical University (The First People's Hospital of Shunde) NO. 1 Jiazi Road, Lunjiao, Shunde District, Foshan City, Guangdong 528308, China. Electronic address: Zhuxlin5@mail2.sysu.edu.cn., Zhang H; State Key Laboratory of Organ Failure Research, Department of Cardiology, Nanfang Hospital, Southern Medical University, Guangzhou, China. Electronic address: zhang12283578@smu.edu.cn., Chen Y; Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China. Electronic address: chenyx39@mail.sysu.edu.cn., Huang X; Department of Cardiology, Shunde Hospital, Southern Medical University (The First People's Hospital of Shunde), NO. 1 Jiazi Road, Lunjiao, Shunde District, Foshan City, Guangdong 528308, China; Medical Research Center, Shunde Hospital, Southern Medical University (The First People's Hospital of Shunde) NO. 1 Jiazi Road, Lunjiao, Shunde District, Foshan City, Guangdong 528308, China. Electronic address: hxhscience@smu.edu.cn., Hu Y; Department of Cardiology, Shunde Hospital, Southern Medical University (The First People's Hospital of Shunde), NO. 1 Jiazi Road, Lunjiao, Shunde District, Foshan City, Guangdong 528308, China; Medical Research Center, Shunde Hospital, Southern Medical University (The First People's Hospital of Shunde) NO. 1 Jiazi Road, Lunjiao, Shunde District, Foshan City, Guangdong 528308, China. Electronic address: hyz.4406@smu.edu.cn., Huang Y; Department of Cardiology, Shunde Hospital, Southern Medical University (The First People's Hospital of Shunde), NO. 1 Jiazi Road, Lunjiao, Shunde District, Foshan City, Guangdong 528308, China; The George Institute for Global Health, Faculty of Medicine, University of New South Wales, Sydney, Australia; Guangdong Provincial Key Laboratory of Cardiac Function and Microcirculation Research, Guangzhou, China; Medical Research Center, Shunde Hospital, Southern Medical University (The First People's Hospital of Shunde) NO. 1 Jiazi Road, Lunjiao, Shunde District, Foshan City, Guangdong 528308, China. Electronic address: hyuli821@smu.edu.cn.
Jazyk: angličtina
Zdroj: Life sciences [Life Sci] 2024 Dec 01; Vol. 358, pp. 123161. Date of Electronic Publication: 2024 Oct 19.
DOI: 10.1016/j.lfs.2024.123161
Abstrakt: Background: Diabetic cardiomyopathy (DCM) is one of the leading causes of death in patients with diabetes mellitus (DM). This study aimed to identify a reliable method for establishing an animal model of DCM for investigation of new targets and treatments.
Methods: Eighty-four 4-week-old male Sprague-Dawley rats were randomly allocated to receive a normal diet or a high-fat diet (HFD) in an approximate ratio of 1:3. At 9 weeks of age, rats in the HFD group received streptozotocin (STZ) 30 mg/kg by intraperitoneal injection and rats in the control group received the same volume of buffer solution. The rodent model of DM was deemed to be successfully established when a random blood glucose measurement was >16.7 mmol/L on three consecutive occasions. If necessary, STZ was readministered.
Results: Three of the 64 rats in the HFD group died after a second STZ injection. DM was induced in 14, 39, and 8 rats after one, two, and three injections, respectively, with cumulative success rates of 21.9 %, 82.8 %, and 95.3 %. Three months later, the rats with DM showed persistent hyperglycemia and insulin resistance and developed histopathological changes indicating cardiac hypertrophy, myocardial fibrosis, and diastolic dysfunction. The metabolic and cardiac histopathological changes were consistent regardless of whether DM was induced by one, two, or three injections of STZ.
Conclusion: An HFD combined with one or more intraperitoneal injections of low-dose STZ is a straightforward and reliable method for inducing DCM in rats. When a single dose of STZ fails to induce DM, repeated injections can be considered.
Competing Interests: Declaration of competing interest The authors declare that they have no competing interests.
(Copyright © 2024 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: