Spinal lumbar Urocortin 3-expressing neurons are associated with both scratching and Compound 48/80-induced sensations.

Autor:	Franck MCM; Department of Immunology, Genetics and Pathology, Uppsala University, Uppsala, Sweden., Weman HM; Department of Immunology, Genetics and Pathology, Uppsala University, Uppsala, Sweden., Ceder MM; Department of Immunology, Genetics and Pathology, Uppsala University, Uppsala, Sweden., Ahemaiti A; Department of Immunology, Genetics and Pathology, Uppsala University, Uppsala, Sweden., Henriksson K; Department of Immunology, Genetics and Pathology, Uppsala University, Uppsala, Sweden., Bengtsson E; Department of Immunology, Genetics and Pathology, Uppsala University, Uppsala, Sweden., Magnusson KA; Department of Immunology, Genetics and Pathology, Uppsala University, Uppsala, Sweden., Koning HK; Department of Immunology, Genetics and Pathology, Uppsala University, Uppsala, Sweden., Öhman-Mägi C; Department of Materials Science and Engineering, Applied Materials Science, Uppsala University, Uppsala, Sweden., Lagerström MC; Department of Immunology, Genetics and Pathology, Uppsala University, Uppsala, Sweden.
Jazyk:	angličtina
Zdroj:	Pain [Pain] 2024 Oct 15. Date of Electronic Publication: 2024 Oct 15.
DOI:	10.1097/j.pain.0000000000003435
Abstrakt:	Abstract: Urocortin 3 is a neuropeptide that belongs to the corticotropin-releasing hormone family and is involved in mechanosensation and stress regulation. In this study, we show that Urocortin 3 marks a population of excitatory neurons in the mouse spinal cord, divided into 2 nonoverlapping subpopulations expressing protein kinase C gamma or calretinin/calbindin 2, populations previously associated with mechanosensation. Electrophysiological experiments demonstrated that lumbar spinal Urocortin 3 neurons receive both glycinergic and GABAergic local tonic inhibition, and monosynaptic inputs from both Aβ and C fibers, which could be confirmed by retrograde trans-synaptic rabies tracing. Furthermore, fos analyses showed that subpopulations of lumbar Urocortin 3 neurons are activated by artificial scratching or Compound 48/80-induced sensations. Chemogenetic activation of lumbar Urocortin 3-Cre neurons evoked a targeted biting/licking behavior towards the corresponding dermatome and chemogenetic inhibition decreased Compound 48/80-induced behavior. Hence, spinal lumbar Urocortin 3 neurons represent a mechanically associated population with roles in both scratching and Compound 48/80-induced sensations. (Copyright © 2024 The Author(s). Published by Wolters Kluwer Health, Inc. on behalf of the International Association for the Study of Pain.)
Databáze:	MEDLINE
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