The leucine-rich repeat receptor kinase QSK1 regulates PRR-RBOHD complexes targeted by the bacterial effector HopF2Pto.

Autor:	Goto Y; Plant Immunity Research Group, RIKEN Center for Sustainable Resource Science (CSRS), Yokohama, Kanagawa 230-0045, Japan.; Graduate School of Science, The University of Tokyo, Tokyo 113-8654, Japan.; Institute of Plant and Microbial Biology, Zurich-Basel Plant Science Center, University of Zurich, Zurich CH-8008, Switzerland., Kadota Y; Plant Immunity Research Group, RIKEN Center for Sustainable Resource Science (CSRS), Yokohama, Kanagawa 230-0045, Japan., Mbengue M; The Sainsbury Laboratory, University of East Anglia, Norwich Research Park, Norwich NR4 7UH, UK.; Laboratoire de Recherche en Sciences Végétales, Université de Toulouse, CNRS, UPS, Toulouse INP, Castanet-Tolosan 31326, France., Lewis JD; Department of Cell and System Biology, Centre for the Analysis of Genome Function and Evolution, University of Toronto, Toronto, ON, Canada M5S 3B2.; Plant Gene Expression, United States Department of Agriculture, Agricultural Research Service, Albany, CA 94710, USA.; Department of Plant and Microbial Biology, University of California Berkeley, Berkeley, CA 94720, USA., Matsui H; Plant Proteomics Research Unit, RIKEN CSRS, Yokohama 230-0045, Japan.; Graduate School of Environmental and Life Science, Okayama University, Okayama 700-8530, Japan., Maki N; Plant Immunity Research Group, RIKEN Center for Sustainable Resource Science (CSRS), Yokohama, Kanagawa 230-0045, Japan., Ngou BPM; Plant Immunity Research Group, RIKEN Center for Sustainable Resource Science (CSRS), Yokohama, Kanagawa 230-0045, Japan., Sklenar J; The Sainsbury Laboratory, University of East Anglia, Norwich Research Park, Norwich NR4 7UH, UK., Derbyshire P; The Sainsbury Laboratory, University of East Anglia, Norwich Research Park, Norwich NR4 7UH, UK., Shibata A; Plant Immunity Research Group, RIKEN Center for Sustainable Resource Science (CSRS), Yokohama, Kanagawa 230-0045, Japan., Ichihashi Y; Plant Immunity Research Group, RIKEN Center for Sustainable Resource Science (CSRS), Yokohama, Kanagawa 230-0045, Japan.; Plant-Microbe Symbiosis Research and Development Team, RIKEN BioResource Research Center, Tsukuba, Ibaraki 305-0074, Japan., Guttman DS; Department of Cell and System Biology, Centre for the Analysis of Genome Function and Evolution, University of Toronto, Toronto, ON, Canada M5S 3B2., Nakagami H; Plant Proteomics Research Unit, RIKEN CSRS, Yokohama 230-0045, Japan.; Protein Mass Spectrometry, Max Planck Institute for Plant Breeding Research, Cologne 50829, Germany., Suzuki T; College of Bioscience and Biotechnology, Chubu University, Kasugai 487-0027, Japan., Menke FLH; The Sainsbury Laboratory, University of East Anglia, Norwich Research Park, Norwich NR4 7UH, UK., Robatzek S; The Sainsbury Laboratory, University of East Anglia, Norwich Research Park, Norwich NR4 7UH, UK.; LMU Biocentre, Ludwig-Maximilian-University of Munich, 82152 Martinsried, Germany., Desveaux D; Department of Cell and System Biology, Centre for the Analysis of Genome Function and Evolution, University of Toronto, Toronto, ON, Canada M5S 3B2., Zipfel C; Institute of Plant and Microbial Biology, Zurich-Basel Plant Science Center, University of Zurich, Zurich CH-8008, Switzerland.; The Sainsbury Laboratory, University of East Anglia, Norwich Research Park, Norwich NR4 7UH, UK., Shirasu K; Plant Immunity Research Group, RIKEN Center for Sustainable Resource Science (CSRS), Yokohama, Kanagawa 230-0045, Japan.; Graduate School of Science, The University of Tokyo, Tokyo 113-8654, Japan.
Jazyk:	angličtina
Zdroj:	The Plant cell [Plant Cell] 2024 Oct 21. Date of Electronic Publication: 2024 Oct 21.
DOI:	10.1093/plcell/koae267
Abstrakt:	Plants detect pathogens using cell-surface pattern recognition receptors (PRRs) such as ELONGATION Factor-TU (EF-TU) RECEPTOR (EFR) and FLAGELLIN SENSING 2 (FLS2), which recognize bacterial EF-Tu and flagellin, respectively. These PRRs belong to the leucine-rich repeat receptor kinase (LRR-RK) family and activate the production of reactive oxygen species via the NADPH oxidase RESPIRATORY BURST OXIDASE HOMOLOG D (RBOHD). The PRR-RBOHD complex is tightly regulated to prevent unwarranted or exaggerated immune responses. However, certain pathogen effectors can subvert these regulatory mechanisms, thereby suppressing plant immunity. To elucidate the intricate dynamics of the PRR-RBOHD complex, we conducted a comparative coimmunoprecipitation analysis using EFR, FLS2, and RBOHD in Arabidopsis thaliana. We identified QIAN SHOU KINASE 1 (QSK1), an LRR-RK, as a PRR-RBOHD complex-associated protein. QSK1 downregulated FLS2 and EFR abundance, functioning as a negative regulator of PRR-triggered immunity (PTI). QSK1 was targeted by the bacterial effector HopF2Pto, a mono-ADP ribosyltransferase, reducing FLS2 and EFR levels through both transcriptional and transcription-independent pathways, thereby inhibiting PTI. Furthermore, HopF2Pto transcriptionally downregulated PROSCOOP genes encoding important stress-regulated phytocytokines and their receptor MALE DISCOVERER 1-INTERACTING RECEPTOR-LIKE KINASE 2. Importantly, HopF2Pto requires QSK1 for its accumulation and virulence functions within plants. In summary, our results provide insights into the mechanism by which HopF2Pto employs QSK1 to desensitize plants to pathogen attack. Competing Interests: Conflict of interest statement. None declared. (© The Author(s) 2024. Published by Oxford University Press on behalf of American Society of Plant Biologists.)
Databáze:	MEDLINE
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