Waveform Optimization for the In Vitro Detection of Caffeic Acid by Fast-Scan Cyclic Voltammetry.
| Autor: | Tonn JN; Department of Chemistry, Roanoke College, 221 College Lane, Salem, Virginia 24153, United States., Keithley RB; Department of Chemistry, Roanoke College, 221 College Lane, Salem, Virginia 24153, United States. |
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| Jazyk: | angličtina |
| Zdroj: | ACS measurement science au [ACS Meas Sci Au] 2024 Jul 31; Vol. 4 (5), pp. 534-545. Date of Electronic Publication: 2024 Jul 31 (Print Publication: 2024). |
| DOI: | 10.1021/acsmeasuresciau.4c00029 |
| Abstrakt: | Caffeic acid is a polyphenol of critical importance in plants, involved in a variety of physiological processes including lignin formation, cellular growth, stress response, and external signaling. This small molecule also acts as a powerful antioxidant and thus has therapeutic potential for a variety of health conditions. Traditional methods of detecting caffeic acid lack appropriate temporal resolution to monitor real time concentration changes on a subsecond time scale with nM detection limits. Here we report on the first usage of fast-scan cyclic voltammetry with carbon fiber microelectrodes for the detection of caffeic acid. Through the use of flow injection analysis, the optimal waveform for its detection under acidic conditions at a scan rate of 400 V/s was determined to be sawtooth-shaped, from 0 to 1.4 to -0.4 to 0 V. Signal was linear with concentration up to 1 μM with a sensitivity of 44.8 ± 1.3 nA/μM and a detection limit of 2.3 ± 0.2 nM. The stability of its detection was exceptional, with an average of 0.96% relative standard deviation across 32 consecutive injections. This waveform was also successful in detecting other catechol-based plant antioxidants including 5-chlorogenic acid, oleuropein, rosmarinic acid, chicoric acid, and caffeic acid phenethyl ester. Finally, we show the successful use of fast-scan cyclic voltammetry in monitoring the degradation of caffeic acid by polyphenol oxidase on a subsecond time scale via a novel modification of a Ramsson cell. This work demonstrates that fast-scan cyclic voltammetry can be used to successfully monitor real-time dynamic changes in the concentrations of catechol-containing plant polyphenols. Competing Interests: The authors declare no competing financial interest. (© 2024 The Authors. Published by American Chemical Society.) |
| Databáze: | MEDLINE |
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