Development of multiplex real-time PCR for simultaneous detection of common fungal pathogens in invasive mycoses.
| Autor: | Ismadi YKM; Department of Medical Microbiology and Parasitology, School of Medical Sciences, Universiti Sains Malaysia, Kubang Kerian, Kelantan, Malaysia., Mohamad S; School of Dental Sciences, Universiti Sains Malaysia, Kubang Kerian, Kelantan, Malaysia., Harun A; Department of Medical Microbiology and Parasitology, School of Medical Sciences, Universiti Sains Malaysia, Kubang Kerian, Kelantan, Malaysia.; Hospital Universiti Sains Malaysia, Universiti Sains Malaysia, Kubang Kerian, Kelantan, Malaysia. |
|---|---|
| Jazyk: | angličtina |
| Zdroj: | PeerJ [PeerJ] 2024 Oct 17; Vol. 12, pp. e18238. Date of Electronic Publication: 2024 Oct 17 (Print Publication: 2024). |
| DOI: | 10.7717/peerj.18238 |
| Abstrakt: | Background: Fungi are common opportunistic pathogens that pose a significant threat to immunocompromised patients, particularly when late detection occurs. Methods: In this study a multiplex real-time PCR has been developed for simultaneous detection of common fungal pathogens associated with invasive mycoses in a diagnostic setting. Results: The specificity of the assay was rigorously tested on 40 types of organisms ( n = 65), demonstrating 100% specificity. The limit of detection was determined to be 100 pg/μl (10 6 copies/μl), achievable within a rapid 3-h timeframe. The PCR assay efficiency exhibited a range between 89.77% and 104.30% for each target organism, with linearity falling between 0.9780 and 0.9983. Conclusion: This multiplex real-time PCR assay holds promise for enhancing the timely and accurate diagnosis of invasive mycoses, particularly in immunocompromised patient populations. Competing Interests: The authors declare that they have no competing interests. (© 2024 Ismadi et al.) |
| Databáze: | MEDLINE |
| Externí odkaz: |
|