Exploring genetic determinants of antimicrobial resistance in Brucella melitensis strains of human and animal origin from India.

Autor:	Ayoub H; Division of Veterinary Public Health, ICAR-Indian Veterinary Research Institute, Izatnagar, India., Kumar MS; Division of Veterinary Public Health, ICAR-Indian Veterinary Research Institute, Izatnagar, India., Mehta R; Division of Veterinary Public Health, ICAR-Indian Veterinary Research Institute, Izatnagar, India., Thomas P; Division of Bacteriology and Mycology, ICAR-Indian Veterinary Research Institute, Izatnagar, India., Dubey M; Xavier University School of Medicine and Xavier University School of Veterinary Medicine, Oranjestad, Aruba., Dhanze H; Division of Veterinary Public Health, ICAR-Indian Veterinary Research Institute, Izatnagar, India., Ajantha GS; Department of Microbiology, SDM College of Medical Sciences and Hospital, Shri Dharmasthala Manjunatheshwara University, Dharwad, India., Bhilegaonkar KN; Division of Veterinary Public Health, ICAR-Indian Veterinary Research Institute, Izatnagar, India., Salih HM; Department of Clinical Sciences, College of Veterinary Medicine, Kansas State University, Manhattan, KS, United States., Cull CA; Midwest Veterinary Services, Inc., Oakland, NE, United States., Veeranna RP; Xavier University School of Medicine and Xavier University School of Veterinary Medicine, Oranjestad, Aruba., Amachawadi RG; Department of Clinical Sciences, College of Veterinary Medicine, Kansas State University, Manhattan, KS, United States.
Jazyk:	angličtina
Zdroj:	Frontiers in microbiology [Front Microbiol] 2024 Oct 04; Vol. 15, pp. 1474957. Date of Electronic Publication: 2024 Oct 04 (Print Publication: 2024).
DOI:	10.3389/fmicb.2024.1474957
Abstrakt:	Introduction: Antimicrobial resistance (AMR) in Brucella melitensis , the causative agent of brucellosis, is of growing concern, particularly in low and middle-income countries. This study aimed to explore the genetic basis of AMR in B. melitensis strains from India. Methods: Twenty-four isolates from humans and animals were subjected to antimicrobial susceptibility testing and whole-genome sequencing. Results: Resistance to doxycycline (20.80%), ciprofloxacin (16.67%), cotrimoxazole (4.17%), and rifampicin (16.67%) was observed. Genome analysis revealed efflux-related genes like mprF , bepG , bepF , bepC , bepE , and bepD across all isolates, however, classical AMR genes were not detected. Mutations in key AMR-associated genes such as rpoB , gyrA , and folP were identified, intriguingly present in both resistant and susceptible isolates, suggesting a complex genotype-phenotype relationship in AMR among Brucella spp. Additionally, mutations in efflux genes were noted in resistant and some susceptible isolates, indicating their potential role in resistance mechanisms. However, mutations in AMR-associated genes did not consistently align with phenotypic resistance, suggesting a multifactorial basis for resistance. Discussion: The study underscores the complexity of AMR in B. melitensis and advocates for a holistic multi-omics approach to fully understand resistance mechanisms. These findings offer valuable insights into genetic markers associated with AMR, guiding future research and treatment strategies. Competing Interests: CC was employed by the Midwest Veterinary Services, Inc. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2024 Ayoub, Kumar, Mehta, Thomas, Dubey, Dhanze, Ajantha, Bhilegaonkar, Salih, Cull, Veeranna and Amachawadi.)
Databáze:	MEDLINE
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