Effect of amikacin-humic acid combination on Acinetobacter baumannii biofilm: an in vitro and in silico study.

Autor:	Rajangam SL; Department of Genetic Engineering, School of Bioengineering, College of Engineering & Technology, SRM Institute of Science & Technology, Kattankulathur, Chennai, Tamil Nadu, 603203, India., Leela KV; Department of Microbiology, SRM Medical College Hospital & Research Centre, SRM Institute of Science & Technology, Kattankulathur, Chennai, Tamil Nadu, 603203, India., Jayaraman M; Department of Microbiology, SRM Medical College Hospital & Research Centre, SRM Institute of Science & Technology, Kattankulathur, Chennai, Tamil Nadu, 603203, India., Sabarathinam S; Pharmaco-Netinformatics Lab, Center for Global Health Research, Saveetha Medical College & Hospitals, Saveetha Institute of Medical & Technical Sciences (SIMATS), Saveetha University, Chennai, Tamil Nadu, 602105, India., Narasimhan MK; Department of Genetic Engineering, School of Bioengineering, College of Engineering & Technology, SRM Institute of Science & Technology, Kattankulathur, Chennai, Tamil Nadu, 603203, India.
Jazyk:	angličtina
Zdroj:	Future microbiology [Future Microbiol] 2024 Oct 21, pp. 1-13. Date of Electronic Publication: 2024 Oct 21.
DOI:	10.1080/17460913.2024.2412431
Abstrakt:	Aim: Acinetobacter baumannii (AB) is a clinically important bacterial pathogen responsible for nosocomial infections. The biofilm-forming capability of these pathogens reduces the antibiotic penetration and its efficacy, thereby complicating the treatment. The current work aims to isolate the most potent biofilm-forming Acinetobacter species from clinical isolates of the patient samples and to evaluate the efficacy of the amikacin-humic acid combination against it. Methods: The combination effect of Amikacin-Humic (AMK-HUM) acid against the highest biofilm-producing A. baumannii SLMK001 was studied via in - vitro (microscopic analysis) and in - silico (Network Pharmacology) analysis. Results: The amikacin-humic acid combination significantly inhibited both the biofilm formation and cell viability of A. baumannii SLMK001. The images observed via Scanning Electron Microscope (SEM) showed a significant decrease in the biofilm matrix. Confocal Laser Scanning Microscope (CLSM) confirmed a reduction of the Z value of its three-dimensional structure. Further, the Network Pharmacology approach supported these experimental findings by identifying the key targets of the amikacin-humic acid combination against the biofilm pathways of A. baumannii . Conclusion: The in-vitro results aligned with the in-silico findings, indicating that the AMK-HUM combination is a promising treatment that significantly activates the key proteins against A. baumannii biofilm formation and pathogenesis.
Databáze:	MEDLINE
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