Association of IL-6 G-174C (rs1800795) variant with the susceptibility to hepatocellular carcinoma in patients with chronic hepatitis.

Autor: Abuelnadar EH; Department of Laboratories, Mansoura University, Children Hospital, Mansoura, Egypt., Ramadan LM; Department of Biochemistry, Biotechnology Research Institute, National Research Centre, Cairo, Egypt., Shahin HE; Nursing Department, College of Applied Medical Sciences, Jouf University, ElQurayyat, Saudi Arabia.; Department of Maternity and Newborn Health Nursing, Health Nursing, Menoufia University, Menoufia, Egypt., Alakilli SYM; Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia., Wahsh E; Department of Pharmacology & Toxicology, Faculty of Pharmacy, Sinai University, Arish Branch, Al-Arish, North Sinai, 45511, Egypt., El-Beltagy NS; Mansoura University Children's Hospital, Mansoura, Egypt., Salem ET; Department of Basic Sciences, Faculty of Physical Therapy, Horus University-Egypt, New Damietta, 34518, Egypt., Hatata AS; Mansoura Manchester Program, Faculty of Medicine, Mansoura University, Mansoura, Egypt., El-Said AM; Genetic Unit, Faculty of Medicine, Mansoura University, Mansoura, Egypt. Afaffahmy21@gmail.com., Alhelf M; Biotechnology School, Nile University, Giza, Egypt.; Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Cairo University, Cairo, Egypt.
Jazyk: angličtina
Zdroj: Journal of the Egyptian National Cancer Institute [J Egypt Natl Canc Inst] 2024 Oct 21; Vol. 36 (1), pp. 32. Date of Electronic Publication: 2024 Oct 21.
DOI: 10.1186/s43046-024-00238-y
Abstrakt: Aim: An ineffective immune response resulting from dysregulation of cytokine production might encourage viral persistence and cause chronic viral hepatitis to worsen. This study examined the relationship between alterations in interleukin-6 (IL-6) levels and the IL-6 - 174 G > C (rs1800795) polymorphism, as well as how this polymorphism affects the development and progression of chronic hepatitis brought on by hepatitis B (HBV) and hepatitis C (HCV) into hepatocellular carcinoma (HCC).
Patients and Methods: Whole blood samples from 126 Egyptian patients with HCC (111 with HCV and 15 with HBV), as well as 126 age- and sex-matched healthy individuals, were used to extract DNA. Using PCR-based allele-specific amplification (ASA), the existence of the IL-6 G-174C polymorphism was investigated. Additionally, each participant's serum IL-6 levels were determined using an enzyme-linked immunosorbent assay (ELISA).
Results: The primary observations revealed that HCC patients had greater serum levels of IL-6 compared to the control groups (p < 0.001). Patients with the variant (CG and GG) genotype in the HCC group were found to have more disease severity indicated by higher levels of alpha-fetoprotein (AFP) and a higher ascites grade, as well as increased inflammatory activity as defined by higher levels of IL-6 and C-reactive protein (CRP) (p < 0.001 for both) in comparison to patients with the wild-type (CC) genotype (p < 0.001 and p = 0.002, respectively).
Conclusion: The rs1800795 SNP in the IL-6 gene was associated with increased inflammatory activity and high levels of IL-6, indicating that this SNP may play a role in the development of HCC in Egyptian patients with chronic viral hepatitis.
(© 2024. The Author(s).)
Databáze: MEDLINE
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