Anti-fatigue effect of an enzymatically derived deer velvet extract through muscle damage recovery and improvement of antioxidant levels.

Autor: Park KH; Yuhan Care Co., Ltd., Yuhan Care R&D Center, Yongin-si, Gyeonggi-do, Republic of Korea; Yuhan Care Co., Ltd., Yuhan Natural Product R&D Center, Andong-si, Gyeongsangbuk-do, Republic of Korea., Park CI; Yuhan Care Co., Ltd., Yuhan Care R&D Center, Yongin-si, Gyeonggi-do, Republic of Korea; Yuhan Care Co., Ltd., Yuhan Natural Product R&D Center, Andong-si, Gyeongsangbuk-do, Republic of Korea., Seo JW; Yuhan Care Co., Ltd., Yuhan Care R&D Center, Yongin-si, Gyeonggi-do, Republic of Korea; Yuhan Care Co., Ltd., Yuhan Natural Product R&D Center, Andong-si, Gyeongsangbuk-do, Republic of Korea., Jeong J; Yuhan Care Co., Ltd., Yuhan Care R&D Center, Yongin-si, Gyeonggi-do, Republic of Korea; Yuhan Care Co., Ltd., Yuhan Natural Product R&D Center, Andong-si, Gyeongsangbuk-do, Republic of Korea., Kim HL; Yuhan Care Co., Ltd., Yuhan Care R&D Center, Yongin-si, Gyeonggi-do, Republic of Korea; Yuhan Care Co., Ltd., Yuhan Natural Product R&D Center, Andong-si, Gyeongsangbuk-do, Republic of Korea., Song A; Yuhan Care Co., Ltd., Yuhan Care R&D Center, Yongin-si, Gyeonggi-do, Republic of Korea; Yuhan Care Co., Ltd., Yuhan Natural Product R&D Center, Andong-si, Gyeongsangbuk-do, Republic of Korea., Park HJ; Yuhan Care Co., Ltd., Yuhan Care R&D Center, Yongin-si, Gyeonggi-do, Republic of Korea; Yuhan Care Co., Ltd., Yuhan Natural Product R&D Center, Andong-si, Gyeongsangbuk-do, Republic of Korea., Cha JY; Yuhan Care Co., Ltd., Yuhan Care R&D Center, Yongin-si, Gyeonggi-do, Republic of Korea; Yuhan Care Co., Ltd., Yuhan Natural Product R&D Center, Andong-si, Gyeongsangbuk-do, Republic of Korea. Electronic address: jycha@yuhancare.com., Kim J; Department of Oral Pathology, School of Dentistry, Jeonbuk National University, Jeonju, Republic of Korea; Non-Clinical Evaluation Center Biomedical Research Institute, Jeonbuk National University Hospital, Jeonju, Republic of Korea. Electronic address: dvmhyun@jbnu.ac.kr.
Jazyk: angličtina
Zdroj: Journal of ethnopharmacology [J Ethnopharmacol] 2025 Jan 30; Vol. 337 (Pt 3), pp. 118965. Date of Electronic Publication: 2024 Oct 18.
DOI: 10.1016/j.jep.2024.118965
Abstrakt: Ethnopharmacological Relevance: Deer velvet (DV) has been extensively used in traditional Oriental medicine to treat various diseases. Its pharmacological spectrum encompasses tonicity, longitudinal bone growth of adolescence, blood retention, hemopoiesis facilitation, enhancement of organ function, physical function improvement, and augmentation of physical vitality. Among its myriad effects, DV notably exhibits anti-fatigue properties; however, its specific mode of action is yet to be fully elucidated.
Aim of the Study: This study was undertaken to investigate the anti-fatigue and exercise performance-enhancing effects of YC-1101(HENKIV®), an enzymatically derived DV extract, in C2C12 cell lines and forced swimming mouse models.
Materials and Methods: The effect of YC-1101 on increasing cell growth and lowering lactate dehydrogenase (LDH) activity was assessed in C2C12. The antioxidative effects of YC-1101 and its mechanistic underpinnings were also evaluated in C2C12 cells. Moreover, mice were subjected to an exhaustive swimming test subsequent to 3 weeks of YC-1101 extract administration. Fatigue-associated biochemical parameters, such as LDH activity and lactate, superoxide dismutase, glutathione peroxidase, and malondialdehyde levels, were measured in serum and muscle tissues.
Results: YC-1101 significantly promoted myoblast growth and reduced LDH activity, indicative of a cell-proliferative effect. Notably, free radical scavenging assays and analysis of reactive oxygen species production and antioxidant-related mRNA expression corroborated the significant involvement of YC-1101 in antioxidation, an important mechanism in anti-fatigue processes. Furthermore, animal experiments demonstrated prolonged swimming endurance and inhibition of muscle LDH accumulation in forced swimming mouse models. Serum biochemical analysis further revealed significant modulation of the expression of anti-fatigue-related biomarkers. Various bioactive low-molecular-weight DV peptides were enriched in YC-1101 compared to YHC-BE-2038 (a DV extract without enzymatic digestion). The anti-fatigue effect of YC-1101 was significantly stronger than that of YHC-BE-2038.
Conclusions: These findings suggest the potential of YC-1101 as a nutraceutical adjunct in ameliorating fatigue, concurrently facilitating muscle damage recovery, and exerting antioxidant effects via the nuclear factor E2-related factor 2-Kelch-like ECH-associated protein-1 pathway. It can be assumed that the complex action of low-molecular-weight DV peptides produced during the enzymatic degradation process was effective, and the further research is needed.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024. Published by Elsevier B.V.)
Databáze: MEDLINE
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