Method validation and determination of pesticides in Mikania glomerta Spreng tincture by direct injection and UPLC-MS/MS analysis.

Autor:	de Brito TM; Laboratório de Farmacologia, Instituto Nacional de Controle de Qualidade em Saúde - FIOCRUZ, Rio de Janeiro, Brazil., de Oliveira AC; Laboratório de Resíduos de Agrotóxicos em Alimentos, Instituto Nacional de Controle de Qualidade em Saúde - FIOCRUZ, Rio de Janeiro, Brazil., Amendoeira FC; Laboratório de Farmacologia, Instituto Nacional de Controle de Qualidade em Saúde - FIOCRUZ, Rio de Janeiro, Brazil., Bastos LHP; Laboratório de Resíduos de Agrotóxicos em Alimentos, Instituto Nacional de Controle de Qualidade em Saúde - FIOCRUZ, Rio de Janeiro, Brazil., Morelli Cardoso MHW; Laboratório de Resíduos de Agrotóxicos em Alimentos, Instituto Nacional de Controle de Qualidade em Saúde - FIOCRUZ, Rio de Janeiro, Brazil., Rocha LM; Laboratório de Tecnologia de Produtos Naturais, Universidade Federal Fluminense, Rio de Janeiro, Brazil., da Nóbrega AW; Laboratório de Resíduos de Agrotóxicos em Alimentos, Instituto Nacional de Controle de Qualidade em Saúde - FIOCRUZ, Rio de Janeiro, Brazil., Ferraris FK; Laboratório de Farmacologia, Instituto Nacional de Controle de Qualidade em Saúde - FIOCRUZ, Rio de Janeiro, Brazil. Electronic address: fausto.ferraris@fiocruz.br.
Jazyk:	angličtina
Zdroj:	Journal of pharmaceutical and biomedical analysis [J Pharm Biomed Anal] 2025 Jan 01; Vol. 252, pp. 116527. Date of Electronic Publication: 2024 Oct 16.
DOI:	10.1016/j.jpba.2024.116527
Abstrakt:	Over the past two decades, concerns have arisen about the efficacy and safety of medicinal plants, highlighting good agricultural practices and quality control. This study aimed to validate a multi-residue analysis method for detecting 268 pesticides in Mikania glomerata tincture, using direct injection and UPLC-MS/MS, per SANTE guidelines. The validation of the method involved evaluating the linearity of analytical curves in terms of the determination coefficient r2 and residuals (%), as well as the limits of quantification (LOQ), matrix effects, precision (expressed as the coefficient of variation, CV), and accuracy (determined as the recovery percentage). The parameters and acceptance criteria were assessed based on SANTE guidelines. The method was then applied to analyse commercial samples of M. glomerata tincture, and traces of carbendazim and dimethomorph were detected. This study underscores the need for regulatory measures to enhance agricultural practices, thereby ensuring the safety and efficacy of medicinal plants. Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze:	MEDLINE
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