Combination of magnetic hyperthermia and gene therapy for breast cancer.

Autor: Solak K; Department of Nanoscience and Nanoengineering, Institute of Science and Technology, Atatürk University, Erzurum, Türkiye.; Department of Molecular Biology and Genetics, Faculty of Science, Erzurum Technical University, Erzurum, Türkiye., Yildiz Arslan S; Department of Molecular Biology and Genetics, Institute of Science and Technology, Atatürk University, Erzurum, Türkiye., Acar M; Department of Molecular Biology and Genetics, Institute of Science and Technology, Atatürk University, Erzurum, Türkiye., Turhan F; Department of Molecular Biology and Genetics, Institute of Science and Technology, Atatürk University, Erzurum, Türkiye., Unver Y; Department of Molecular Biology and Genetics, Faculty of Science, Atatürk University, Erzurum, Türkiye. yunver@atauni.edu.tr., Mavi A; Department of Nanoscience and Nanoengineering, Institute of Science and Technology, Atatürk University, Erzurum, Türkiye. amavi@atauni.edu.tr.; Department of Mathematics and Science Education, Education Faculty of Kazim Karabekir, Atatürk University, Erzurum, Türkiye. amavi@atauni.edu.tr.
Jazyk: angličtina
Zdroj: Apoptosis : an international journal on programmed cell death [Apoptosis] 2024 Oct 19. Date of Electronic Publication: 2024 Oct 19.
DOI: 10.1007/s10495-024-02026-4
Abstrakt: This study presented a novel breast cancer therapy model that uses magnetic field-controlled heating to trigger gene expression in cancer cells. We created silica- and amine-modified superparamagnetic nanoparticles (MSNP-NH 2 ) to carry genes and release heat under an alternating current (AC) magnetic field. The heat-inducible expression plasmid (pHSP-Azu) was designed to encode anti-cancer azurin and was delivered by magnetofection. MCF-7 cells demonstrated over 93% cell viability and 12% transfection efficiency when exposed to 75 µg/ml of MSNP-NH 2 , 3 µg of DNA, and PEI at a 0.75 PEI/DNA ratio (w: w), unlike non-tumorigenic cells (MCF-10 A). Magnetic hyperthermia (MHT) increased azurin expression by heat induction, leading to cell death in dual ways. The combination of MHT and heat-regulated azurin expression induced cell death, specifically in cancer cells, while having negligible effects on MCF-10 A cells. The proposed strategy clearly shows that simultaneous use of MHT and MHT-induced azurin gene expression may selectively target and kill cancer cells, offering a promising direction for cancer therapy.
(© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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