The In vitro evaluation of in silico-designed synthetic peptides AKVUAM-1 and AKVUAM-2 on human lung fibroblast cell line MRC5 and Mycobacterium tuberculosis isolates.

Autor: Simsek E; Department of Nutrition and Dietetics, Institute of Health Sciences, Akdeniz University, Antalya, Turkey; Department of Medical Biotechnology, Institute of Health Sciences, Akdeniz University, Antalya, Turkey. Electronic address: ecesimsek@akdeniz.edu.tr., Yildirim K; Department of Nutrition and Dietetics, Institute of Health Sciences, Akdeniz University, Antalya, Turkey; Department of Medical Biotechnology, Institute of Health Sciences, Akdeniz University, Antalya, Turkey., Akcit ET; Department of Medical Biotechnology, Institute of Health Sciences, Akdeniz University, Antalya, Turkey; Department of Medical Services and Techniques, Dialysis Program, Vocational School of Health Services, Akdeniz University, Antalya, Turkey., Atas C; Department of Medical Biotechnology, Institute of Health Sciences, Akdeniz University, Antalya, Turkey., Kocak O; Department of Biology, Institute of Natural and Applied Sciences, Akdeniz University, Antalya, Turkey., Altinkaynak A; Department of Nutrition and Dietetics, Institute of Health Sciences, Akdeniz University, Antalya, Turkey., Salehi Moharer MP; Faculty of Medicine, Akdeniz University, Antalya, Turkey., Yazici E; Department of Medical Biotechnology, Institute of Health Sciences, Akdeniz University, Antalya, Turkey., Sisaneci A; Department of Medical Biotechnology, Institute of Health Sciences, Akdeniz University, Antalya, Turkey., Kalay M; Department of Histology and Embryology, Faculty of Medicine, Akdeniz University, Antalya, Turkey., Tanriover G; Department of Medical Biotechnology, Institute of Health Sciences, Akdeniz University, Antalya, Turkey; Department of Histology and Embryology, Faculty of Medicine, Akdeniz University, Antalya, Turkey., Uzun M; Department of Medical Microbiology, Istanbul Medical School, Istanbul University, Istanbul, Turkey., Coban AY; Department of Nutrition and Dietetics, Institute of Health Sciences, Akdeniz University, Antalya, Turkey; Department of Medical Biotechnology, Institute of Health Sciences, Akdeniz University, Antalya, Turkey.
Jazyk: angličtina
Zdroj: Microbial pathogenesis [Microb Pathog] 2024 Dec; Vol. 197, pp. 107027. Date of Electronic Publication: 2024 Oct 18.
DOI: 10.1016/j.micpath.2024.107027
Abstrakt: Tuberculosis is a major global health problem caused by Mycobacterium tuberculosis and the increase in drug resistance is driving the need for new treatments. Today, various approaches are being applied in the development of drugs for the treatment of tuberculosis. Computer-aided drug design (CADD) enables the prediction of pharmacological efficacy for potential drug molecules during the design process. Thus, new therapeutic compounds can be developed that are more potent, less toxic and have fewer side effects than existing drugs. In this study, we investigated the in vitro activities of AKVUAM-1 and AKVUAM-2 synthetic peptides designed in silico by computer-aided drug design method to inhibit the interaction between M. tuberculosis outer membrane protein Cpn T and macrophage surface receptor CR-1 and Surfactant D protein. Notably, these synthetic peptides do not show cytotoxic effect on normal lung tissue and do not kill M. tuberculosis directly. The MIC values for AKVUAM-1 were higher than 512 μg/ml for all bacterial strains except IST-16 strain (128 μg/ml). According to our results, AKVUAM-1 and AKVUAM-2 synthetic peptides have the potential to be successful candidates for investigating their potential to block macrophage entry of bacilli as targeted.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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