Alternative splicing and deletion in S-RNase confer stylar-part self-compatibility in the apple cultivar 'Vered'.

Autor: Okada K; Institute of Fruit Tree and Tea Science, National Agriculture and Food Research Organization, 2-1 Fujimoto, Tsukuba, Ibaraki, 305-8605, Japan. okak@affrc.go.jp., Shimizu T; Division of Apple Research, Institute of Fruit Tree and Tea Science, National Agriculture and Food Research Organization, 92-24 Nabeyashiki, Shimokuriyagawa, Morioka, Iwate, 020-0123, Japan., Moriya S; Division of Apple Research, Institute of Fruit Tree and Tea Science, National Agriculture and Food Research Organization, 92-24 Nabeyashiki, Shimokuriyagawa, Morioka, Iwate, 020-0123, Japan., Wada M; Division of Apple Research, Institute of Fruit Tree and Tea Science, National Agriculture and Food Research Organization, 92-24 Nabeyashiki, Shimokuriyagawa, Morioka, Iwate, 020-0123, Japan., Abe K; Division of Apple Research, Institute of Fruit Tree and Tea Science, National Agriculture and Food Research Organization, 92-24 Nabeyashiki, Shimokuriyagawa, Morioka, Iwate, 020-0123, Japan., Sawamura Y; Division of Apple Research, Institute of Fruit Tree and Tea Science, National Agriculture and Food Research Organization, 92-24 Nabeyashiki, Shimokuriyagawa, Morioka, Iwate, 020-0123, Japan.
Jazyk: angličtina
Zdroj: Plant molecular biology [Plant Mol Biol] 2024 Oct 19; Vol. 114 (6), pp. 113. Date of Electronic Publication: 2024 Oct 19.
DOI: 10.1007/s11103-024-01514-0
Abstrakt: Although self-incompatibility in apples (Malus × domestica Borkh.) is regulated by a single S-locus with multiple S-haplotypes that comprise pistil S (S-RNase) and pollen S genes, it is not desirable in commercial orchards because it requires cross-pollination to achieve stable fruit production. Therefore, it is important to identify and characterize self-compatible apple cultivars. However, little is known about self-compatibility (SC) and its underlying molecular mechanisms in apples. In this study, we discovered that 'Vered', an early maturing and low chilling-requiring apple cultivar, exhibits stable SC, which was evaluated via self-pollination tests. The S-genotype of 'Vered' was designated as S 24 S 39 sm . Results of genetic analysis of selfed progeny of 'Vered' revealed that SC is associated with the S 39 sm -haplotype, and molecular analyses indicated that it is caused by alternative splicing and a 205-bp deletion in S 39 sm -RNase. These events induce frameshifts and ultimately produce the defective S 39 sm -RNase isoforms that lack their C-terminal half. These results enabled us to develop a 117-bp DNA marker that can be used to assist in the selection of self-compatible apples with the dysfunctional S 39 sm -RNase. Thus, analysis of 'Vered' provided insights into the molecular mechanism of the very rare trait of natural stylar-part SC. Moreover, 'Vered' is a valuable genetic resource for breeding cultivars with SC and/or low chilling requirement in apple. Our findings contribute to a better understanding of self-compatible molecular mechanisms in apple and provide for the accelerated breeding of self-compatible apple cultivars.
(© 2024. The Author(s), under exclusive licence to Springer Nature B.V.)
Databáze: MEDLINE
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