| Autor: |
Tindula G; Department of Epidemiology and Population Health, Stanford University, Stanford, CA, USA., Mukherjee SK; Department of Paediatric Neurosurgery, National Institute of Neurosciences and Hospital (NINS&H), Dhaka, Bangladesh., Ekramullah SM; Department of Paediatric Neurosurgery, National Institute of Neurosciences and Hospital (NINS&H), Dhaka, Bangladesh., Arman DM; Department of Paediatric Neurosurgery, National Institute of Neurosciences and Hospital (NINS&H), Dhaka, Bangladesh., Islam J; Department of Clinical Neurosurgery, National Institute of Neurosciences and Hospital (NINS&H), Dhaka, Bangladesh., Biswas SK; Department of Molecular and Cell Biology, University of Connecticut, Storrs, CT, USA., Warf BC; Department of Neurosurgery, Boston Children's Hospital, Boston, MA, USA., Christiani DC; Department of Environmental Health, Harvard T.H. Chan School of Public Health, Boston, MA, USA., Lemos B; Department of Environmental Health, Harvard T.H. Chan School of Public Health, Boston, MA, USA.; Department of Pharmacology and Toxicology, R. Ken Coit College of Pharmacy, The University of Arizona, Tucson, AZ, USA.; Coit Center for Longevity and Neurotherapeutics, The University of Arizona, Tucson, AZ, USA., Liang L; Department of Epidemiology, Harvard T.H. Chan School of Public Health, Boston, MA, USA.; Department of Biostatistics, Harvard T.H. Chan School of Public Health, Boston, MA, USA., Cardenas A; Department of Epidemiology and Population Health, Stanford University, Stanford, CA, USA., Mazumdar M; Department of Environmental Health, Harvard T.H. Chan School of Public Health, Boston, MA, USA.; Department of Neurology, Boston Children's Hospital, Boston, MA, USA.; Department of Neurology, Harvard Medical School, Boston, MA, USA. |
| Abstrakt: |
An emerging hypothesis linking arsenic toxicity involves altered epigenetic mechanisms, such as DNA methylation. In this study, we examined the relationship between parents' arsenic exposure and DNA methylation in tissues obtained from 28 infants with spina bifida from Bangladesh. We analyzed arsenic in parents' toenails using inductively coupled plasma mass spectrometry (ICP-MS). DNA methylation was measured in infants' dural tissue, buccal swabs, and whole blood using the Illumina Infinium MethylationEPIC BeadChip. We performed epigenome-wide association analyses (EWAS) and tested differentially methylated regions (DMRs). In EWAS, DNA methylation at cg24039697 in dural tissue was positively associated (β = 0.59, p = 7.6 × 10 -9 ) with father's toenail arsenic concentrations, adjusting for covariates. We did not identify any CpG sites related to father's arsenic exposure in the other tissues, or any CpG sites related to mother's arsenic exposure. Gene ontology analysis identified many biological pathways of interest, including the Wnt signaling pathways. We identified several DMRs across the tissues related to arsenic exposure that included probes mapping to genes that have previously been identified in studies of neural tube defects. This study emphasizes the potential impact of arsenic exposure in fathers, often understudied in epidemiological studies, on DNA methylation in a unique neurological tissue specific to spina bifida. |
