The Sperm-Associated Antigen 11A (Spag11a) Knockout Mice Display Sub-Fertility and Perturbations in the Sperm Proteome.

Autor: Sangeeta K; Department of Animal Biology, University of Hyderabad, Hyderabad, India., Aisha J; Department of Animal Biology, University of Hyderabad, Hyderabad, India., Yenugu S; Department of Animal Biology, University of Hyderabad, Hyderabad, India.
Jazyk: angličtina
Zdroj: Cell biochemistry and function [Cell Biochem Funct] 2024 Sep; Vol. 42 (7), pp. e70003.
DOI: 10.1002/cbf.70003
Abstrakt: Spermatogenesis and sperm maturation that occur in the testis and epididymis respectively are crucial for fertility. Factors secreted by the testicular and epididymal epithelial cells govern the processes of gametogenesis and maturation. Proteins encoded by the sperm-associated antigen 11a (Spag11a) gene are implicated as having a possible role in sperm maturation. However, studies that demonstrate their definite role in fertility and sperm function using knockout models have not yet reported. In this study, Spag11a knockout mice were generated, genotyped and the reproductive parameters (fecundity, sperm count, capacitation, and acrosome reaction) and sperm proteome were determined. Litter size and sperm count were decreased in the Spag11a knockout mice when compared to the wild-type controls. Spermatozoa from the knockout mice were able to undergo capacitation. However, acrosome reaction did not occur in sperm obtained from knockout mice. Structural abnormalities in the head and tail structures were evident in the spermatozoa of knockout mice. Perturbations in the expression of sperm proteins that are involved in gametogenesis were evident. The subfertility observed in Spag11a knockout mice could be a manifestation of lower sperm count, impaired acrosome reactions, and disturbances in the sperm proteome. The results of this study lend further support to the role of Spag11a gene in male gamete function.
(© 2024 John Wiley & Sons Ltd.)
Databáze: MEDLINE
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