Whole genome sequencing of multidrug-resistant Klebsiella pneumoniae from poultry in Noakhali, Bangladesh: Assessing risk of transmission to humans in a pilot study.

Autor: Munim MA; Department of Biotechnology and Genetic Engineering, Noakhali Science and Technology University, Noakhali 3814, Bangladesh. Electronic address: adnanbge.nstu@gmail.com., Tanni AA; Department of Genetic Engineering and Biotechnology, University of Chittagong, Chattogram 4331, Bangladesh; Next Generation Sequencing, Research and Innovation Laboratory Chattogram (NRICh), Biotechnology Research and Innovation Centre (BRIC), University of Chittagong, Chattogram, Bangladesh. Electronic address: afrozatanni.geb@cu.ac.bd., Hossain MM; International Centre for Diarrhoeal Disease Research (iccdr,b), Bangladesh. Electronic address: parvej.geb.cu@gmail.com., Chakma K; Department of Genetic Engineering and Biotechnology, University of Chittagong, Chattogram 4331, Bangladesh; Next Generation Sequencing, Research and Innovation Laboratory Chattogram (NRICh), Biotechnology Research and Innovation Centre (BRIC), University of Chittagong, Chattogram, Bangladesh. Electronic address: kallyan679@gmail.com., Mannan A; Department of Genetic Engineering and Biotechnology, University of Chittagong, Chattogram 4331, Bangladesh; Next Generation Sequencing, Research and Innovation Laboratory Chattogram (NRICh), Biotechnology Research and Innovation Centre (BRIC), University of Chittagong, Chattogram, Bangladesh. Electronic address: adnan.mannan@cu.ac.bd., Islam SMR; Department of Genetic Engineering and Biotechnology, University of Chittagong, Chattogram 4331, Bangladesh; Next Generation Sequencing, Research and Innovation Laboratory Chattogram (NRICh), Biotechnology Research and Innovation Centre (BRIC), University of Chittagong, Chattogram, Bangladesh. Electronic address: drsmrafiqcu@gmail.com., Tiwari JG; School of Veterinary Medicine, Murdoch University, 90 South St, Murdoch, WA 6150, Australia. Electronic address: Jully.GogoiTiwari@murdoch.edu.au., Gupta SD; Department of Biotechnology and Genetic Engineering, Noakhali Science and Technology University, Noakhali 3814, Bangladesh. Electronic address: shipan.bge@nstu.edu.bd.
Jazyk: angličtina
Zdroj: Comparative immunology, microbiology and infectious diseases [Comp Immunol Microbiol Infect Dis] 2024 Nov; Vol. 114, pp. 102246. Date of Electronic Publication: 2024 Oct 10.
DOI: 10.1016/j.cimid.2024.102246
Abstrakt: Background: Multi-drug resistant (MDR) Klebsiella pneumoniae is a public health concern due to its presence in Bangladeshi poultry products and its ability to spread resistance genes. This study genetically characterizes a distinct MDR K. pneumoniae isolate from the gut of poultry in Noakhali, Bangladesh, offering insights into its resistance mechanisms and public health impact.
Methods: Klebsiella pneumoniae isolates from broiler and layer poultry were identified using biochemical and molecular analyses. Eleven isolates were tested for antibiotic sensitivity and categorized by their Multiple Antibiotic Resistance Index (MARI) profiles. The isolate with the highest MARI was selected for whole-genome sequencing using Illumina technology. The sequencing data were analyzed for genome annotation, pan-genome analysis, genome similarities, sequence type identification, and the identification of genetic determinants of resistance and virulence genes.
Result: We identified 10 MARI profiles among 11 K. pneumoniae isolates, with values ranging from 0.64 to 0.94. The highest MARI of 0.94 was found in an isolate from a layer poultry. This isolate's genome, 5401,789 base pairs long with 89.6 % coverage, showed potential inter-species dissemination, as indicated by core genome phylogenetic analysis. It possessed genes conferring resistance to fluoroquinolones, aminoglycosides, β-lactams, folate pathway antagonists, fosfomycin, macrolides, quinolones, rifamycin, tetracyclines, and polymyxins, including colistin.
Conclusion: Poultry serve as reservoirs for MDR K. pneumoniae, which can spread to other species and pose significant health risks. Rigorous monitoring of antibiotic use and genetic characterization of MDR bacterial isolates are essential to mitigate this threat.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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