How do bacteria tune transcription termination efficiency?

Autor: Julia Dierksheide K; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA., Battaglia RA; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA., Li GW; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA. Electronic address: gwli@mit.edu.
Jazyk: angličtina
Zdroj: Current opinion in microbiology [Curr Opin Microbiol] 2024 Dec; Vol. 82, pp. 102557. Date of Electronic Publication: 2024 Oct 17.
DOI: 10.1016/j.mib.2024.102557
Abstrakt: Bacterial operons often contain intergenic transcription terminators that terminate some, but not all, RNA polymerase molecules. In these operons, the level of terminator readthrough determines downstream gene expression and helps establish protein ratios among co-regulated genes. Despite its critical role in maintaining stoichiometric gene expression, terminator strength remains difficult to predict from DNA sequence. The necessary features of a major class of bacterial terminators - intrinsic terminators - have been known for half a century, but a strong sequence-function model has yet to be developed. Here, we summarize high-throughput approaches for probing the sequence determinants of intrinsic termination efficiency and discuss the impact of trans-acting factors on this sequence-function relationship. Building on the main lessons from these studies, we map out the experimental challenges that must be circumvented to establish a quantitative model for termination efficiency.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Author(s). Published by Elsevier Ltd.. All rights reserved.)
Databáze: MEDLINE
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