| Autor: |
Lee MJ; Kimchi Functionality Research Group, World Institute of Kimchi, Gwangju 61755, Republic of Korea.; Department of Life Science, Chung-Ang University, Seoul 06974, Republic of Korea., Yun JU; Kimchi Functionality Research Group, World Institute of Kimchi, Gwangju 61755, Republic of Korea.; Department of Life Science, Chosun University, Gwangju 61452, Republic of Korea., Nam S; Kimchi Functionality Research Group, World Institute of Kimchi, Gwangju 61755, Republic of Korea.; Department of Integrative Biological Sciences, Chosun University, Gwangju 61452, Republic of Korea., Jung MJ; Kimchi Functionality Research Group, World Institute of Kimchi, Gwangju 61755, Republic of Korea., Kim YB; Kimchi Functionality Research Group, World Institute of Kimchi, Gwangju 61755, Republic of Korea., Kim Y; Kimchi Functionality Research Group, World Institute of Kimchi, Gwangju 61755, Republic of Korea., Lee J; Kimchi Functionality Research Group, World Institute of Kimchi, Gwangju 61755, Republic of Korea., Choi DG; Department of Life Science, Chung-Ang University, Seoul 06974, Republic of Korea., Lee DS; Department of Life Science, Chung-Ang University, Seoul 06974, Republic of Korea., Roh SW; Microbiome Research Team, LISCure Biosciences Inc., Seongnam 13486, Republic of Korea., Whon TW; Kimchi Functionality Research Group, World Institute of Kimchi, Gwangju 61755, Republic of Korea., Jeon CO; Department of Life Science, Chung-Ang University, Seoul 06974, Republic of Korea., Lee SH; Kimchi Functionality Research Group, World Institute of Kimchi, Gwangju 61755, Republic of Korea. |
| Abstrakt: |
The novel strain CBA3628 T was isolated from kimchi, a Korean fermented vegetable. CBA3628 T is a cocci-shaped, Gram-stain-positive, catalase- and oxidase-negative and facultatively anaerobic bacterium. The results of phylogenetic analysis based on 16S rRNA gene sequencing indicated that CBA3628 T represented a member of the genus Leuconostoc of the family Leuconostocaceae . CBA3628 T has a circular chromosomal genome and three plasmids of 1 864 558 bp (37% DNA G+C content), containing 1,887 genes, 1,762 predicted protein-coding genes, 4 complete rRNA loci and 70 tRNA genes. The cells were non-haemolytic, non-motile and non-spore forming. The optimal growth of CBA3628 T occurred at 30 °C, pH 6.0 and with 0-2% (w/v) NaCl. The major polar lipids of CBA3628 T were diphosphatidylglycerol and phosphatidylglycerol. The major fatty acids (>10%) of CBA3628 T were C 16 : 0 , C 20 : 0 and C 19 : 0 cyclo ω8 c . CBA3628 T contained A3α-type peptidoglycans. CBA3628 T was most closely related to Leuconostoc mesenteroides subsp. mesenteroides ATCC 8293 T , L. mesenteroides subsp. dextranicum DSM 20484 T and L. suionicum DSM 20241 T with 99.52% 16S rRNA gene sequence similarity. However, the average nucleotide identities of 91.9%, 91.7% and 91.1% and the digital DNA-DNA hybridisation values of 45.6%, 45.4% and 45.4% indicated that the novel isolate represented a distinct species. Phylogenetic analyses of both the 16S rRNA gene and genome sequences revealed that CBA3628 T formed a distinct phylogenetic lineage within the genus Leuconostoc and was most closely related to Leuconostoc litchii MB7 T . The ANI and dDDH values between CBA3628 T and L. litchii MB7 T were 84.9 and 22.8%, respectively. Functional genes belonging to COG categories E, J and K were enriched in the genome of CBA3628 T (>7.9%). On the basis of its physiological, chemotaxonomic, phylogenetic and genomic properties, strain CBA3628 T represents a novel species from the genus Leuconostoc , for which we propose the name Leuconostoc koreense sp. nov., with the type strain CBA3628 T (= KACC 23049 T = DSM 116836 T ). |
