| Autor: |
Lazarchuk P; Department of Laboratory Medicine and Pathology, University of Washington, Seattle, WA 98195, USA., Nguyen MM; Department of Laboratory Medicine and Pathology, University of Washington, Seattle, WA 98195, USA.; Present address: Fred Hutchinson Cancer Center, Seattle, WA 98109, USA., Curca CM; Department of Laboratory Medicine and Pathology, University of Washington, Seattle, WA 98195, USA.; Present address: Parse Biosciences, Seattle, WA 98109, USA., Pavlova MN; Department of Laboratory Medicine and Pathology, University of Washington, Seattle, WA 98195, USA., Oshima J; Department of Laboratory Medicine and Pathology, University of Washington, Seattle, WA 98195, USA., Sidorova JM; Department of Laboratory Medicine and Pathology, University of Washington, Seattle, WA 98195, USA. |
| Abstrakt: |
Werner syndrome of premature aging is caused by mutations in the WRN RECQ helicase/exonuclease, which functions in DNA replication, repair, transcription, and telomere maintenance. How the loss of WRN accelerates aging is not understood in full. Here we show that WRN is necessary for optimal constitutive heterochromatin levels in proliferating human fibroblasts. Locally, WRN deficiency derepresses SATII pericentromeric satellite repeats but does not reduce replication fork progression on SATII repeats. Globally, WRN loss reduces a subset of protein-protein interactions responsible for the organization of constitutive heterochromatin in the nucleus, namely, the interactions involving Lamin B1 and Lamin B receptor, LBR. Both the mRNA level and subcellular distribution of LBR are affected by WRN deficiency, and unlike the former, the latter phenotype does not require WRN catalytic activities. The phenotypes of heterochromatin disruption seen in WRN-deficient proliferating fibroblasts are also observed in WRN-proficient fibroblasts undergoing replicative or oncogene-induced senescence. WRN interacts with histone deacetylase 2, HDAC2; WRN/HDAC2 association is mediated by heterochromatin protein alpha, HP1α, and WRN complexes with HP1α and HDAC2 are downregulated in senescing cells. The data suggest that the effect of WRN loss on heterochromatin is separable from senescence program, but mimics at least some of the heterochromatin changes associated with it. |
