Metagenomic Detection of Multiple Viruses in Monk Parakeet (Myiopsitta monachus) in Australia.

Autor: Kanti Nath B; School of Agricultural, Environmental and Veterinary Sciences, Faculty of Science and Health, Charles Sturt University, Wagga Wagga, New South Wales, Australia.; Biosecurity Research Program and Training Centre, Gulbali Institute, Charles Sturt University, Wagga Wagga, New South Wales, Australia., Gupta SD; School of Agricultural, Environmental and Veterinary Sciences, Faculty of Science and Health, Charles Sturt University, Wagga Wagga, New South Wales, Australia.; Biosecurity Research Program and Training Centre, Gulbali Institute, Charles Sturt University, Wagga Wagga, New South Wales, Australia., Talukder S; College of Public Health, Medical and Veterinary Sciences, James Cook University, Townsville, Queensland, Australia., Tonu NS; Upazila Livestock Office and Veterinary Hospital, Cumilla, Bangladesh., Raidal SR; School of Agricultural, Environmental and Veterinary Sciences, Faculty of Science and Health, Charles Sturt University, Wagga Wagga, New South Wales, Australia., Forwood JK; Biosecurity Research Program and Training Centre, Gulbali Institute, Charles Sturt University, Wagga Wagga, New South Wales, Australia.; School of Dentistry and Medical Sciences, Faculty of Science and Health, Charles Sturt University, Wagga Wagga, New South Wales, Australia., Sarker S; Biomedical Sciences & Molecular Biology, College of Public Health, Medical and Veterinary Sciences, James Cook University, Townsville, Queensland, Australia.; Australian Institute of Tropical Health and Medicine, James Cook University, Townsville, Queensland, Australia.; Department of Microbiology, Anatomy, Physiology, and Pharmacology, School of Agriculture, Biomedicine and Environment, La Trobe University, Melbourne, Victoria, Australia.
Jazyk: angličtina
Zdroj: Veterinary medicine and science [Vet Med Sci] 2024 Nov; Vol. 10 (6), pp. e70083.
DOI: 10.1002/vms3.70083
Abstrakt: Background: Birds are known to harbour many pathogens, including circovirus, herpesviruses, adenoviruses and Chlamydia psittaci. Some of these pose zoonotic risks, while others, such as beak and feather disease virus (BFDV), have a significant impact on the conservation of endangered bird species.
Objectives: This study was aimed to determine the faecal virome of a group of apparently healthy Monk parakeet using high-throughput sequencing.
Methods: Fresh faecal samples were collected from four Monk parakeets at a pet shop in Melbourne, Australia. Virus enrichment and nucleic acid extraction were performed on the faecal samples, followed by high-throughput sequencing at the Australian Genome Research Facility (AGRF).
Results: Utilising an established pipeline for high-throughput sequencing data analysis, this study revealed the presence of three viruses of the families Circoviridae, Parvoviridae and Adenoviridae. Subsequent sequence comparison and phylogenetic analyses further confirmed that the detected viruses belong to the genera Chaphamaparvovirus (unassigned species), Circovirus (species Circovirus parrot) and Siadenovirus (species Siadenovirus viridis).
Conclusion: Despite non-pathogenicity, the existence of multiple viruses within a bird species underscores the risk of these viruses spreading into the pet trade. Detection and a better understanding of avian viruses are crucial for the establishment of appropriate management and biosecurity measures in the domestic and international bird trade, which ultimately supports the conservation of vulnerable bird species.
(© 2024 The Author(s). Veterinary Medicine and Science published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
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