Identification of potential auxin response candidate genes for soybean rapid canopy coverage through comparative evolution and expression analysis.

Autor:	Ferreira Neres D; Biological Systems Engineering, Virginia Polytechnic Institute and State University, Blacksburg, VA, United States.; Translational Plant Science Center, Virginia Polytechnic Institute and State University, Blacksburg, VA, United States., Taylor JS; Translational Plant Science Center, Virginia Polytechnic Institute and State University, Blacksburg, VA, United States.; School of Plant and Environmental Sciences, Virginia Polytechnic Institute and State University, Blacksburg, VA, United States., Bryant JA Jr; Biological Systems Engineering, Virginia Polytechnic Institute and State University, Blacksburg, VA, United States.; Translational Plant Science Center, Virginia Polytechnic Institute and State University, Blacksburg, VA, United States., Bargmann BOR; Translational Plant Science Center, Virginia Polytechnic Institute and State University, Blacksburg, VA, United States.; School of Plant and Environmental Sciences, Virginia Polytechnic Institute and State University, Blacksburg, VA, United States., Wright RC; Biological Systems Engineering, Virginia Polytechnic Institute and State University, Blacksburg, VA, United States.; Translational Plant Science Center, Virginia Polytechnic Institute and State University, Blacksburg, VA, United States.
Jazyk:	angličtina
Zdroj:	Frontiers in plant science [Front Plant Sci] 2024 Oct 03; Vol. 15, pp. 1463438. Date of Electronic Publication: 2024 Oct 03 (Print Publication: 2024).
DOI:	10.3389/fpls.2024.1463438
Abstrakt:	Introduction: Throughout domestication, crop plants have gone through strong genetic bottlenecks, dramatically reducing the genetic diversity in today's available germplasm. This has also reduced the diversity in traits necessary for breeders to develop improved varieties. Many strategies have been developed to improve both genetic and trait diversity in crops, from backcrossing with wild relatives, to chemical/radiation mutagenesis, to genetic engineering. However, even with recent advances in genetic engineering we still face the rate limiting step of identifying which genes and mutations we should target to generate diversity in specific traits. Methods: Here, we apply a comparative evolutionary approach, pairing phylogenetic and expression analyses to identify potential candidate genes for diversifying soybean (Glycine max) canopy cover development via the nuclear auxin signaling gene families, while minimizing pleiotropic effects in other tissues. In soybean, rapid canopy cover development is correlated with yield and also suppresses weeds in organic cultivation. Results and Discussion: We identified genes most specifically expressed during early canopy development from the TIR1/AFB auxin receptor, Aux/IAA auxin co-receptor, and ARF auxin response factor gene families in soybean, using principal component analysis. We defined Arabidopsis thaliana and model legume species orthologs for each soybean gene in these families allowing us to speculate potential soybean phenotypes based on well-characterized mutants in these model species. In future work, we aim to connect genetic and functional diversity in these candidate genes with phenotypic diversity in planta allowing for improvements in soybean rapid canopy cover, yield, and weed suppression. Further development of this and similar algorithms for defining and quantifying tissue- and phenotype-specificity in gene expression may allow expansion of diversity in valuable phenotypes in important crops. Competing Interests: DF and RW have filed an invention disclosure related to the algorithm developed in this work. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2024 Ferreira Neres, Taylor, Bryant, Bargmann and Wright.)
Databáze:	MEDLINE
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