Pegylated gold nanoparticles interact with lipid bilayer and human serum albumin and transferrin.

Autor: Okła E; Faculty of Biology and Environmental Protection, Department of General Biophysics, University of Lodz, Pomorska 141/143, Lodz, 90-236, Poland. elzbieta.okla@biol.uni.lodz.pl.; University of Lodz Doctoral School of Exact and Natural Sciences, 21/23 Matejki St., Lodz, 90-237, Poland. elzbieta.okla@biol.uni.lodz.pl., Michlewska S; Faculty of Biology and Environmental Protection, Laboratory of Microscopic Imaging and Specialized Biological Techniques, University of Lodz, Banacha 12/16, Lodz, 90-237, Poland., Buczkowski A; Faculty of Chemistry, Department of Physical Chemistry, Division of Biophysical Chemistry, University of Lodz, Pomorska 165, Lodz, 90-236, Poland., Zawadzki S; Faculty of Biology and Environmental Protection, Department of General Biophysics, University of Lodz, Pomorska 141/143, Lodz, 90-236, Poland.; BioMedChem Doctoral School of the University of Lodz and Lodz Institutes of the Polish Academy of Sciences, 21/23 Matejki St., 90‑237 , Lodz, Poland., Miłowska K; Faculty of Biology and Environmental Protection, Department of General Biophysics, University of Lodz, Pomorska 141/143, Lodz, 90-236, Poland., Sánchez-Nieves J; Universidad de Alcalá Department of Organic and Inorganic Chemistry, and Research Institute in Chemistry 'Andrés M. del Río' (IQAR), Spain and Instituto Ramon y Cajal de Investigacion Sanitaria, IRYCIS, Colmenar Viejo Road, Km 9, 100, Madrid, 28034, Spain.; Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Madrid, Spain., Gómez R; Universidad de Alcalá Department of Organic and Inorganic Chemistry, and Research Institute in Chemistry 'Andrés M. del Río' (IQAR), Spain and Instituto Ramon y Cajal de Investigacion Sanitaria, IRYCIS, Colmenar Viejo Road, Km 9, 100, Madrid, 28034, Spain.; Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Madrid, Spain., de la Mata FJ; Universidad de Alcalá Department of Organic and Inorganic Chemistry, and Research Institute in Chemistry 'Andrés M. del Río' (IQAR), Spain and Instituto Ramon y Cajal de Investigacion Sanitaria, IRYCIS, Colmenar Viejo Road, Km 9, 100, Madrid, 28034, Spain.; Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Madrid, Spain., Bryszewska M; Faculty of Biology and Environmental Protection, Department of General Biophysics, University of Lodz, Pomorska 141/143, Lodz, 90-236, Poland., Blasiak J; Collegium Medicum, Faculty of Medicine, Mazovian Academy in Plock, Pl. Dabrowskiego 2, Plock, 09-402, Poland., Ionov M; Faculty of Biology and Environmental Protection, Department of General Biophysics, University of Lodz, Pomorska 141/143, Lodz, 90-236, Poland. maksim.ionov@biol.uni.lodz.pl.; Collegium Medicum, Faculty of Medicine, Mazovian Academy in Plock, Pl. Dabrowskiego 2, Plock, 09-402, Poland. maksim.ionov@biol.uni.lodz.pl.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2024 Oct 18; Vol. 14 (1), pp. 24408. Date of Electronic Publication: 2024 Oct 18.
DOI: 10.1038/s41598-024-74898-0
Abstrakt: Gold nanoparticles (AuNPs) are potentially applicable in drug/nucleic acid delivery systems. Low toxicity, high stability, and bioavailability are crucial for the therapeutic use of AuNPs and they are mainly determined by their interactions with proteins and lipids on their route to the target cells. In this work, we investigated the interaction of two pegylated gold nanoparticles, AuNP14a and AuNP14b, with human serum proteins albumin (HSA) and transferrin (Tf) as well as dimyristoyl-phosphatidylcholine (DMPC) liposomes, which can be a representative of biomembranes. We showed that AuNP14a/b interacted with HSA and Tf changing their electrical, thermodynamic, and structural properties as evidenced by dynamic light scattering, zeta potential, transmission electron microscopy, circular dichroism, fluorescence quenching, and isothermal titration calorimetry. These nanoparticles penetrated the DMPC membrane suggesting their ability to reach a target inside the cell. In most of the effects, AuNP14b was more effective than AuNP14a, which might result from its more positive charge. Further studies are needed to evaluate whether the interaction of AuNP14a/b with HSA and Tf is safe for the cell/organism and whether they may safely penetrate natural membranes.
(© 2024. The Author(s).)
Databáze: MEDLINE
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