Maternal and neonatal factors' effects on wharton's jelly mesenchymal stem cell yield.

Autor: Mahmoud R; Pediatric Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt., Bassiouny M; Pediatric Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt., Badawy A; Obstetric and Gynecology Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt., Darwish A; Pediatric Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt. ahmaddarwish@mans.edu.eg.; Mansoura Research Center for Cord Stem Cells (MARC-CSC), Faculty of Medicine, Mansoura University, Mansoura, Egypt. ahmaddarwish@mans.edu.eg., Yahia S; Pediatric Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt., El-Tantawy N; Medical Parasitology Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2024 Oct 17; Vol. 14 (1), pp. 24376. Date of Electronic Publication: 2024 Oct 17.
DOI: 10.1038/s41598-024-72386-z
Abstrakt: As Wharton's jelly-derived mesenchymal stem cells (WJ-MSCs) are easily accessible, easy to isolate, and ethically acceptable, they represent a promising source of MSCs for use in regenerative medicine. Considering decisions on WJ-MSCs collection requires extensive knowledge of the factors that impact their yield. This study's aim was to evaluate the influence of parameters related to mothers and newborns on the WJ-MSCs yield. The WJ-MSCs were isolated and expanded after being isolated from 79 umbilical cord (UC) samples. Population doubling time and cell proliferation were assessed. By flow cytometry analysis, WJ-MSCs were identified by positivity of CD105, CD90, and CD73 and negativity of CD45 and CD34. There was a statistically significant negative correlation between UC width and P1 doubling time. Maternal age and WJ-MSC yield were shown to be negatively correlated. Birth weight and gestational age showed a significant positive correlation between WJ-MSCs yield and neonatal variables. No significant correlations were detected between the WJ-MSCs and the mother parity, nor the neonatal sex, fetal presentation, or head circumference. The WJ-MSCs yield increases with younger maternal age, higher gestational age, and increased neonatal birth weight. Hence, consideration should be given to these factors when selecting the ideal donors.
(© 2024. The Author(s).)
Databáze: MEDLINE
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